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Science 31 May 1985:
Vol. 228. no. 4703, pp. 1094 - 1096
DOI: 10.1126/science.2986291
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Articles

Science, Vol 228, Issue 4703, 1094-1096
Copyright © 1985 by American Association for the Advancement of Science

articles

Virus envelope protein of HTLV-III represents major target antigen for antibodies in AIDS patients

F Barin, MF McLane, JS Allan, TH Lee, JE Groopman, and M Essex

In this study, two glycoproteins (gp160 and gp120) that are encoded by human T-cell lymphoma virus type III (HTLV-III) were the antigens most consistently recognized by antibodies found in patients with the acquired immune deficiency syndrome (AIDS) and with the AIDS-related complex (ARC) and in healthy homosexual males. The techniques used to detect the glycoproteins were radioimmunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (RIP/SDS-PAGE). Although most antibody-positive samples from ARC patients and from healthy homosexual males also reacted with the virus core protein p24, less than half of the AIDS patients revealed a positive band with p24 under the same conditions. The ability to detect antibodies against a profile of both the major env gene encoded antigens and the gag gene encoded antigens suggests that the RIP/SDS-PAGE may be a valuable confirmatory assay for establishing the presence or absence of antibodies to HTLV-III in human serum samples.


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Ann Intern Med 104, 194-196
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Antigens of Human T-Lymphotropic Virus Type III/Lymphadenopathy-Associated Virus.
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Characterization of gp41 as the transmembrane protein coded by the HTLV-III/LAV envelope gene.
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Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III.
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Major glycoprotein antigens that induce antibodies in AIDS patients are encoded by HTLV-III.
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