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Science 31 May 1985:
Vol. 228. no. 4703, pp. 1061 - 1068
DOI: 10.1126/science.2986289
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Articles

Science, Vol 228, Issue 4703, 1061-1068
Copyright © 1985 by American Association for the Advancement of Science

articles

Chromatin structure and de novo methylation of sperm DNA: implications for activation of the paternal genome

M Groudine and KF Conkin

The chromatin structure characteristic of constitutively expressed genes, tissue-specific genes, and inactive genes is absent in chicken sperm chromatin. However, point sites of undermethylation in sperm DNA within constitutively expressed genes, but not within globin genes or an inactive gene, correspond to the location of regions of altered chromatin structure (hypersensitive sites) in somatic tissue and spermatogonial cells. A de novo methylation process whereby regions within and flanking these genes become methylated, but which excludes the methylation of sequences within hypersensitive sites, occurs between the spermatogonial stage and the first meiotic prophase. These undermethylated regions may play a role in the activation of the paternal genome during embryogenesis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
DNA Methylation Density Influences the Stability of an Epigenetic Imprint and Dnmt3a/b-Independent De Novo Methylation.
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Deoxyribonucleic Acid Hypomethylation of Male Germ Cells by Mitotic and Meiotic Exposure to 5-Azacytidine Is Associated with Altered Testicular Histology.
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Heterogeneous Expression of the SSX Cancer/Testis Antigens in Human Melanoma Lesions and Cell Lines.
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ATP-dependent reorganization of human sperm nuclear chromatin.
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Enhanced transcription of c-myc in bursal lymphoma cells requires continuous protein synthesis.
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Nuclear Transfer in Mouse Embryos: Activation of the Embryonic Genome.
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