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Science 17 May 1985:
Vol. 228. no. 4701, pp. 889 - 891
DOI: 10.1126/science.3890180
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Articles

Science, Vol 228, Issue 4701, 889-891
Copyright © 1985 by American Association for the Advancement of Science

articles

High-affinity uptake of serotonin into immunocytochemically identified astrocytes

HK Kimelberg and DM Katz

Primary cultures of astrocytes from neonatal rat brain were incubated with tritiated serotonin. After fixation they were stained by immunofluorescence for the astrocyte-specific marker glial fibrillary acidic protein and processed for autoradiography. Silver grain density was increased over cells positive for glial fibrillary acidic protein and was reduced to background levels when sodium was omitted from the medium or the specific inhibitors of serotonin uptake fluoxetine and chlorimipramine were present. The results indicate that mammalian astrocytes can take up serotonin by a sodium-dependent, high-affinity system previously thought to be the exclusive property of serotonergic nerve endings.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Autoregulation of Serotonin Neurons: Role in Antidepressant Drug Action.
G. Pineyro and P. Blier (1999)
Pharmacol. Rev. 51, 533-591
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