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Science 10 May 1985:
Vol. 228. no. 4700, pp. 742 - 744
DOI: 10.1126/science.2581315
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Articles

Science, Vol 228, Issue 4700, 742-744
Copyright © 1985 by American Association for the Advancement of Science

articles

Expression of the chlamydial genus-specific lipopolysaccharide epitope in Escherichia coli

FE Nano and HD Caldwell

The obligate intracellular prokaryote Chlamydia trachomatis is the etiological agent of trachoma and is a primary causative pathogen of sexually transmitted genital tract disease; both diseases affect millions of people each year. The cloning of genes encoding the enzyme or enzymes producing the genus-specific lipopolysaccharide antigen of Chlamydia into Escherichia coli is reported here. The cloned chlamydial lipopolysaccharide antigen appears to be a hybrid lipopolysaccharide molecule composed of both Chlamydia and Escherichia coli components. The chlamydial lipopolysaccharide antigen is expressed on the surfaces of the viable Escherichia coli recombinants. These findings may have a significant impact on defining the role of this highly conserved antigen in the pathogenesis and diagnosis of chlamydial infections.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Kdo-(2 -> 8)-Kdo-(2 -> 4)-Kdo but not Kdo-(2 -> 4)-Kdo-(2 -> 4)-Kdo is an acceptor for transfer of L-glycero-{alpha}-D-manno-heptose by Escherichia coli heptosyltransferase I (WaaC).
S. Gronow, B. Lindner, H. Brade, and S. Muller-Loennies (2009)
Innate Immunity 15, 13-23
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A monoclonal antibody against a carbohydrate epitope in lipopolysaccharide differentiates Chlamydophila psittaci from Chlamydophila pecorum, Chlamydophila pneumoniae, and Chlamydia trachomatis.
S. Muller-Loennies, S. Gronow, L. Brade, R. MacKenzie, P. Kosma, and H. Brade (2006)
Glycobiology 16, 184-196
   Abstract »    Full Text »    PDF »
A monoclonal antibody recognizing the 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) trisaccharide {alpha}Kdo(2->4){alpha}Kdo(2->4){alpha}Kdo of Chlamydophila spacepsittaci 6BC lipopolysaccharide.
L. Brade, A. Rozalski, P. Kosma, and H. Brade (2000)
Innate Immunity 6, 361-368
   Abstract »    PDF »
Structural Analysis of the Lipopolysaccharide from Chlamydia trachomatis Serotype L2.
S. Rund, B. Lindner, H. Brade, and O. Holst (1999)
J. Biol. Chem. 274, 16819-16824
   Abstract »    Full Text »    PDF »
Characterization of a Neutralizing Monoclonal Antibody Directed at the Lipopolysaccharide of Chlamydia pneumoniae.
E. M. Peterson, L. M. de la Maza, L. Brade, and H. Brade (1998)
Infect. Immun. 66, 3848-3855
   Abstract »    Full Text »    PDF »
Review: Chlamydial lipopolysaccharide.
H. Brade, W. Brabetz, L. Brade, O. Hoist, S. Lobau, M. Lucakova, U. Mamat, A. Rozalski, K. Zych, and P. Kosma (1997)
Innate Immunity 4, 67-84
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