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Science 10 May 1985:
Vol. 228. no. 4700, pp. 740 - 742
DOI: 10.1126/science.2581314
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Articles

Science, Vol 228, Issue 4700, 740-742
Copyright © 1985 by American Association for the Advancement of Science

articles

Identification of human glucocorticoid receptor complementary DNA clones by epitope selection

C Weinberger, SM Hollenberg, ES Ong, JM Harmon, ST Brower, J Cidlowski, EB Thompson, MG Rosenfeld, and RM Evans

Steroid hormones regulate cellular differentiation and physiologic functions predominantly through gene transcription. Regulation is achieved by the interaction of specific steroid receptor proteins and target genes. Expression cloning techniques were used to select human glucocorticoid receptor complementary DNA clones in order to define the mechanism by which the receptor exerts its transcriptional control. Immobilized fusion proteins from individual clones were used to select epitope-specific antibody which was subsequently eluted and identified by binding to protein blots of cellular extracts. Three cross-hybridizing clones containing inserts expressing antigenic determinants of the human glucocorticoid receptor were isolated.


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