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Science 3 May 1985:
Vol. 228. no. 4699, pp. 546 - 553
DOI: 10.1126/science.3983639
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Articles

Science, Vol 228, Issue 4699, 546-553
Copyright © 1985 by American Association for the Advancement of Science

articles

Crystallographic structure of the octameric histone core of the nucleosome at a resolution of 3.3 A

RW Burlingame, WE Love, BC Wang, R Hamlin, HX Nguyen, and EN Moudrianakis

The structure of the (H2A-H2B-H3-H4)2 histone octamer has been determined by means of x-ray crystallographic techniques at a resolution of 3.3 angstroms. The octamer is a prolate ellipsoid 110 angstroms long and 65 to 70 angstroms in diameter, and its general shape is that of a rugby ball. The size and shape are radically different from those determined in earlier studies. The most striking feature of the histone octamer is its tripartite organization, that is, a central (H3-H4)2 tetramer flanked by two H2A-H2B dimers. The DNA helix, placed around the octamer in a path suggested by the features on the surface of the protein, appears like a spring holding the H2A-H2B dimers at either end of the (H3-H4)2 tetramer.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Microscopic DNA Flexibility Analysis. PROBING THE BASE COMPOSITION AND ION DEPENDENCE OF MINOR GROOVE COMPRESSION WITH AN ARTIFICIAL DNA BENDING AGENT.
T. Akiyama and M. E. Hogan (1996)
J. Biol. Chem. 271, 29126-29135
   Abstract »    Full Text »    PDF »
Antigen Specificity of Antihistone Antibodies in Localized Scleroderma.
S. Sato, M. Fujimoto, H. Ihn, K. Kikuchi, and K. Takehara (1994)
Arch Dermatol 130, 1273-1277
   Abstract »    PDF »
Studies of Nucleosome Structure.
T.J. Richmond, T. Rechsteiner, and K. Luger (1993)
Cold Spring Harb Symp Quant Biol 58, 265-272
   Abstract »    PDF »
How Eco RI recognizes and cuts DNA.
H. Berman (1986)
Science 234, 1482-1483
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Shape analysis of the histone octamer in solution.
E. Uberbacher, J. Harp, E Wilkinson-Singley, and G. Bunick (1986)
Science 232, 1247-1249
   Abstract »    PDF »
Crystallographic structure of the octamer histone core of the nucleosome.
A KLUG, J. FINCH, and T. RICHMOND (1985)
Science 229, 1109-1113
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Crystallographic Structure of the Octamer Histone Core of the Nucleosome.
E. N. MOUDRIANAKIS, W. E. LOVE, B. C. WANG, N. G. XUONG, and R. W. BURLINGAME (1985)
Science 229, 1110-1112
   PDF »
Crystallographic Structure of the Octamer Histone Core of the Nucleosome.
E. C. UBERBACHER and G. J. BUNICK (1985)
Science 229, 1112-1113
   PDF »
Crystallographic Structure of the Octamer Histone Core of the Nucleosome.
E. N. MOUDRIANAKIS, W. E. LOVE, and R. W. BURLINGAME (1985)
Science 229, 1113
   PDF »


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