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Science 8 March 1985:
Vol. 227. no. 4691, pp. 1233 - 1235
DOI: 10.1126/science.227.4691.1233
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Articles

Monoclonal Antibodies Against the Aster Yellows Agent

CHAN-PIN LIN 1 and TSEH AN CHEN 1

1 Department of Plant Pathology, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick 08903

Hybridoma clones secreting specific monoclonal antibodies against the aster yellows agent, a mycoplasma-like organism, were produced by using partially purified salivary gland preparations from infected leafhopper vectors as the immunogen. After 3947 hybridomas from 20 independent fusions were screened for specific antibody against the aster yellows agent, two table clones were obtained. With these monoclonal antibodies the aster yellows agent in diseased lettuce, periwinkles, and inoculative insects was specifically identified by enzyme-linked immunosorbent assay. The aster yellows agent was serologically differentiated from the mycoplasma-like organisms associated with ash yellows, loofah witches'-broom, paulownia witches'-broom, sweet potato witches'-broom, peanut rosette, maize bushy stunt, and elm phloem necrosis.

Submitted on October 15, 1984
Accepted on November 8, 1984


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