Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
CareStream

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 8 March 1985:
Vol. 227. no. 4691, pp. 1229 - 1231
DOI: 10.1126/science.227.4691.1229
Prev | Table of Contents | Next

Articles

A Simple and General Method for Transferring Genes into Plants

Transformed petunia, tobacco, and tomato plants have been produced by means of a novel leaf disk transformation-regeneration method. Surface-sterilized leaf disks were inoculated with an Agrobacterium tumefaciens strain containing a modified tumor-inducing plasmid (in which the phytohormone biosynthetic genes from transferred DNA had been deleted and replaced with a chimeric gene for kanamycin resistance) and cultured for 2 days. The leaf disks were then transferred to selective medium containing kanamycin. Shoot regeneration occurred within 2 to 4 weeks, and transformants were confirmed by their ability to form roots in medium containing kanamycin. This method for producing transformed plants combines gene transfer, plant regeneration, and effective selection for transformants into a single process and should be applicable to plant species that can be infected by Agrobacterium and regenerated from leaf explants.

Submitted on October 1, 1984
Accepted on November 19, 1984


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Promoter of a cotton fibre MYB gene functional in trichomes of Arabidopsis and glandular trichomes of tobacco.
X.-X. Shangguan, B. Xu, Z.-X. Yu, L.-J. Wang, and X.-Y. Chen (2008)
J. Exp. Bot. 59, 3533-3542
   Abstract »    Full Text »    PDF »
Overexpression of the Arabidopsis 10-Kilodalton Acyl-Coenzyme A-Binding Protein ACBP6 Enhances Freezing Tolerance.
Q.-F. Chen, S. Xiao, and M.-L. Chye (2008)
Plant Physiology 148, 304-315
   Abstract »    Full Text »    PDF »
Patterning of Inflorescences and Flowers by the F-Box Protein DOUBLE TOP and the LEAFY Homolog ABERRANT LEAF AND FLOWER of Petunia.
E. Souer, A. B. Rebocho, M. Bliek, E. Kusters, R. A.M. de Bruin, and R. Koes (2008)
PLANT CELL 20, 2033-2048
   Abstract »    Full Text »    PDF »
Overexpression of phytochelatin synthase in tobacco: distinctive effects of AtPCS1 and CePCS genes on plant response to cadmium.
S. Wojas, S. Clemens, J. Hennig, A. Sklodowska, E. Kopera, H. Schat, W. Bal, and D. M. Antosiewicz (2008)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
The Scientific Roots of Modern Plant Biotechnology.
I. M. Sussex (2008)
PLANT CELL 20, 1189-1198
   Full Text »    PDF »
Cloning and characterization of the UDP-glucose:anthocyanin 5-O-glucosyltransferase gene from blue-flowered gentian.
T. Nakatsuka, K. Sato, H. Takahashi, S. Yamamura, and M. Nishihara (2008)
J. Exp. Bot. 59, 1241-1252
   Abstract »    Full Text »    PDF »
SodERF3, a Novel Sugarcane Ethylene Responsive Factor (ERF), Enhances Salt and Drought Tolerance when Overexpressed in Tobacco Plants.
L. E. Trujillo, M. Sotolongo, C. Menendez, M. E. Ochogavia, Y. Coll, I. Hernandez, O. Borras-Hidalgo, B. P. H. J. Thomma, P. Vera, and L. Hernandez (2008)
Plant Cell Physiol. 49, 512-525
   Abstract »    Full Text »    PDF »
A Plasma Membrane-Anchored Fluorescent Protein Fusion Illuminates Sieve Element Plasma Membranes in Arabidopsis and Tobacco.
M. V. Thompson and S. M. Wolniak (2008)
Plant Physiology 146, 1599-1610
   Abstract »    Full Text »    PDF »
CYP725A4 from Yew Catalyzes Complex Structural Rearrangement of Taxa-4(5),11(12)-diene into the Cyclic Ether 5(12)-Oxa-3(11)-cyclotaxane.
D. Rontein, S. Onillon, G. Herbette, A. Lesot, D. Werck-Reichhart, C. Sallaud, and A. Tissier (2008)
J. Biol. Chem. 283, 6067-6075
   Abstract »    Full Text »    PDF »
Analysis of GDP-D-Mannose Pyrophosphorylase Gene Promoter from Acerola (Malpighia glabra) and Increase in Ascorbate Content of Transgenic Tobacco Expressing the Acerola Gene.
A. A. Badejo, N. Tanaka, and M. Esaka (2008)
Plant Cell Physiol. 49, 126-132
   Abstract »    Full Text »    PDF »
pSAT RNA Interference Vectors: A Modular Series for Multiple Gene Down-Regulation in Plants.
M. Dafny-Yelin, S.-M. Chung, E. L. Frankman, and T. Tzfira (2007)
Plant Physiology 145, 1272-1281
   Abstract »    Full Text »    PDF »
A Universal Expression/Silencing Vector in Plants.
Y. Peretz, R. Mozes-Koch, F. Akad, E. Tanne, H. Czosnek, and I. Sela (2007)
Plant Physiology 145, 1251-1263
   Abstract »    Full Text »    PDF »
Polypeptide Substrate Specificity of PsLSMT: A SET DOMAIN PROTEIN METHYLTRANSFERASE.
R. Magnani, N. R. Nayak, M. Mazarei, L. M. A. Dirk, and R. L. Houtz (2007)
J. Biol. Chem. 282, 27857-27864
   Abstract »    Full Text »    PDF »
Artificial MicroRNA-Mediated Virus Resistance in Plants.
J. Qu, J. Ye, and R. Fang (2007)
J. Virol. 81, 6690-6699
   Abstract »    Full Text »    PDF »
Isolation and characterization of a Vitis vinifera transcription factor, VvWRKY1, and its effect on responses to fungal pathogens in transgenic tobacco plants.
C. Marchive, R. Mzid, L. Deluc, F. Barrieu, J. Pirrello, A. Gauthier, M.-F. Corio-Costet, F. Regad, B. Cailleteau, S. Hamdi, et al. (2007)
J. Exp. Bot. 58, 1999-2010
   Abstract »    Full Text »    PDF »
Control of cell and petal morphogenesis by R2R3 MYB transcription factors.
K. Baumann, M. Perez-Rodriguez, D. Bradley, J. Venail, P. Bailey, H. Jin, R. Koes, K. Roberts, and C. Martin (2007)
Development 134, 1691-1701
   Abstract »    Full Text »    PDF »
Expression of the Human Hepatitis B Virus Large Surface Antigen Gene in Transgenic Tomato Plants.
X.-M. Lou, Q.-H. Yao, Z. Zhang, R.-H. Peng, A.-S. Xiong, and H.-K. Wang (2007)
Clin. Vaccine Immunol. 14, 464-469
   Abstract »    Full Text »    PDF »
A truncated MYB transcription factor from Antirrhinum majus regulates epidermal cell outgrowth.
F. W. Jaffe, A. Tattersall, and B. J. Glover (2007)
J. Exp. Bot. 58, 1515-1524
   Abstract »    Full Text »    PDF »
Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity.
S. Schaarschmidt, M.-C. Gonzalez, T. Roitsch, D. Strack, U. Sonnewald, and B. Hause (2007)
Plant Physiology 143, 1827-1840
   Abstract »    Full Text »    PDF »
MAP Kinases Function Downstream of HSP90 and Upstream of Mitochondria in TMV Resistance Gene N-Mediated Hypersensitive Cell Death.
R. Takabatake, Y. Ando, S. Seo, S. Katou, S. Tsuda, Y. Ohashi, and I. Mitsuhara (2007)
Plant Cell Physiol. 48, 498-510
   Abstract »    Full Text »    PDF »
Channeling of Eukaryotic Diacylglycerol into the Biosynthesis of Plastidial Phosphatidylglycerol.
M. Fritz, H. Lokstein, D. Hackenberg, R. Welti, M. Roth, U. Zahringer, M. Fulda, W. Hellmeyer, C. Ott, F. P. Wolter, et al. (2007)
J. Biol. Chem. 282, 4613-4625
   Abstract »    Full Text »    PDF »
Transgenic induction of mitochondrial rearrangements for cytoplasmic male sterility in crop plants.
A. P. S. Sandhu, R. V. Abdelnoor, and S. A. Mackenzie (2007)
PNAS 104, 1766-1770
   Abstract »    Full Text »    PDF »
Expression of a Novel Small Antimicrobial Protein from the Seeds of Motherwort (Leonurus japonicus) Confers Disease Resistance in Tobacco.
X. Yang, Y. Xiao, X. Wang, and Y. Pei (2007)
Appl. Envir. Microbiol. 73, 939-946
   Abstract »    Full Text »    PDF »
Characterization of two ethylene receptors PhERS1 and PhETR2 from petunia: PhETR2 regulates timing of anther dehiscence.
Y. Wang and P. P. Kumar (2007)
J. Exp. Bot. 58, 533-544
   Abstract »    Full Text »    PDF »
Characterization of a Novel Plant Promoter Specifically Induced by Heavy Metal and Identification of the Promoter Regions Conferring Heavy Metal Responsiveness.
X. Qi, Y. Zhang, and T. Chai (2007)
Plant Physiology 143, 50-59
   Abstract »    Full Text »    PDF »
Colloquium Paper: Artificial evolution extends the spectrum of viruses that are targeted by a disease-resistance gene from potato.
G. Farnham and D. C. Baulcombe (2006)
PNAS 103, 18828-18833
   Abstract »    Full Text »    PDF »
Multiple virus resistance at a high frequency using a single transgene construct.
E. Bucher, D. Lohuis, P. M. J. A. van Poppel, C. Geerts-Dimitriadou, R. Goldbach, and M. Prins (2006)
J. Gen. Virol. 87, 3697-3701
   Abstract »    Full Text »    PDF »
Arbuscular mycorrhiza induces gene expression of the apoplastic invertase LIN6 in tomato (Lycopersicon esculentum) roots.
S. Schaarschmidt, T. Roitsch, and B. Hause (2006)
J. Exp. Bot. 57, 4015-4023
   Abstract »    Full Text »    PDF »
Overexpression of an Arabidopsis magnesium transport gene, AtMGT1, in Nicotiana benthamiana confers Al tolerance.
W. Deng, K. Luo, D. Li, X. Zheng, X. Wei, W. Smith, C. Thammina, L. Lu, Y. Li, and Y. Pei (2006)
J. Exp. Bot. 57, 4235-4243
   Abstract »    Full Text »    PDF »
Reduction of Benzenoid Synthesis in Petunia Flowers Reveals Multiple Pathways to Benzoic Acid and Enhancement in Auxin Transport.
I. Orlova, A. Marshall-Colon, J. Schnepp, B. Wood, M. Varbanova, E. Fridman, J. J. Blakeslee, W. A. Peer, A. S. Murphy, D. Rhodes, et al. (2006)
PLANT CELL 18, 3458-3475
   Abstract »    Full Text »    PDF »
Transgenic Tobacco Plants Overexpressing Chitinases of Fungal Origin Show Enhanced Resistance to Biotic and Abiotic Stress Agents.
M. d. l. M. Dana, J. A. Pintor-Toro, and B. Cubero (2006)
Plant Physiology 142, 722-730
   Abstract »    Full Text »    PDF »
Accumulation of Recombinant SARS-CoV Spike Protein in Plant Cytosol and Chloroplasts Indicate Potential for Development of Plant-Derived Oral Vaccines.
H.-Y. Li, S. Ramalingam, and M.-L. Chye (2006)
Experimental Biology and Medicine 231, 1346-1352
   Abstract »    Full Text »    PDF »
The Nightshade Proteinase Inhibitor IIb Gene is Constitutively Expressed in Glandular Trichomes.
J. Liu, K.-F. Xia, J.-C. Zhu, Y.-G. Deng, X.-L. Huang, B.-L. Hu, X. Xu, and Z.-F. Xu (2006)
Plant Cell Physiol. 47, 1274-1284
   Abstract »    Full Text »    PDF »
The TATA-Box Sequence in the Basal Promoter Contributes to Determining Light-Dependent Gene Expression in Plants.
K. Kiran, S. A. Ansari, R. Srivastava, N. Lodhi, C. P. Chaturvedi, S. V. Sawant, and R. Tuli (2006)
Plant Physiology 142, 364-376
   Abstract »    Full Text »    PDF »
Plant-Produced Cottontail Rabbit Papillomavirus L1 Protein Protects against Tumor Challenge: a Proof-of-Concept Study..
T. Kohl, I. I. Hitzeroth, D. Stewart, A. Varsani, V. A. Govan, N. D. Christensen, A.-L. Williamson, and E. P. Rybicki (2006)
Clin. Vaccine Immunol. 13, 845-853
   Abstract »    Full Text »    PDF »
Analysis of the Petunia TM6 MADS Box Gene Reveals Functional Divergence within the DEF/AP3 Lineage.
A. S. Rijpkema, S. Royaert, J. Zethof, G. van der Weerden, T. Gerats, and M. Vandenbussche (2006)
PLANT CELL 18, 1819-1832
   Abstract »    Full Text »    PDF »
Cosecretion of Protease Inhibitor Stabilizes Antibodies Produced by Plant Roots.
S. Komarnytsky, N. Borisjuk, N. Yakoby, A. Garvey, and I. Raskin (2006)
Plant Physiology 141, 1185-1193
   Abstract »    Full Text »    PDF »
Endogenous and Synthetic MicroRNAs Stimulate Simultaneous, Efficient, and Localized Regulation of Multiple Targets in Diverse Species.
J. P. Alvarez, I. Pekker, A. Goldshmidt, E. Blum, Z. Amsellem, and Y. Eshed (2006)
PLANT CELL 18, 1134-1151
   Abstract »    Full Text »    PDF »
Control of the Synthesis and Subcellular Targeting of the Two GDH Genes Products in Leaves and Stems of Nicotiana plumbaginifolia and Arabidopsis thaliana.
J.-X. Fontaine, F. Saladino, C. Agrimonti, M. Bedu, T. Terce-Laforgue, T. Tetu, B. Hirel, F. M. Restivo, and F. Dubois (2006)
Plant Cell Physiol. 47, 410-418
   Abstract »    Full Text »    PDF »
A Front-end Desaturase from Chlamydomonas reinhardtii Produces Pinolenic and Coniferonic Acids by {omega}13 Desaturation in Methylotrophic Yeast and Tobacco.
M. Kajikawa, K. T. Yamato, Y. Kohzu, S.-i. Shoji, K. Matsui, Y. Tanaka, Y. Sakai, and H. Fukuzawa (2006)
Plant Cell Physiol. 47, 64-73
   Abstract »    Full Text »    PDF »
Colonization by the Arbuscular Mycorrhizal Fungus Glomus versiforme Induces a Defense Response Against the Root-knot Nematode Meloidogyne incognita in the Grapevine (Vitis amurensis Rupr.), Which Includes Transcriptional Activation of the Class III Chitinase Gene VCH3.
H.-Y. Li, G.-D. Yang, H.-R. Shu, Y.-T. Yang, B.-X. Ye, I. Nishida, and C.-C. Zheng (2006)
Plant Cell Physiol. 47, 154-163
   Abstract »    Full Text »    PDF »
Expression Profiling Identifies Genes Expressed Early During Lint Fibre Initiation in Cotton.
Y. Wu, A. C. Machado, R. G. White, D. J. Llewellyn, and E. S. Dennis (2006)
Plant Cell Physiol. 47, 107-127
   Abstract »    Full Text »    PDF »
RDR6 Has a Broad-Spectrum but Temperature-Dependent Antiviral Defense Role in Nicotiana benthamiana.
F. Qu, X. Ye, G. Hou, S. Sato, T. E. Clemente, and T. J. Morris (2005)
J. Virol. 79, 15209-15217
   Abstract »    Full Text »    PDF »
A Mitogen-activated Protein Kinase NtMPK4 Activated by SIPKK is Required for Jasmonic Acid Signaling and Involved in Ozone Tolerance via Stomatal Movement in Tobacco.
K. Gomi, D. Ogawa, S. Katou, H. Kamada, N. Nakajima, H. Saji, T. Soyano, M. Sasabe, Y. Machida, I. Mitsuhara, et al. (2005)
Plant Cell Physiol. 46, 1902-1914
   Abstract »    Full Text »    PDF »
The products of the broken Tm-2 and the durable Tm-22 resistance genes from tomato differ in four amino acids.
F. C. Lanfermeijer, J. Warmink, and J. Hille (2005)
J. Exp. Bot. 56, 2925-2933
   Abstract »    Full Text »    PDF »
Conversion of nicotine to nornicotine in Nicotiana tabacum is mediated by CYP82E4, a cytochrome P450 monooxygenase.
B. Siminszky, L. Gavilano, S. W. Bowen, and R. E. Dewey (2005)
PNAS 102, 14919-14924
   Abstract »    Full Text »    PDF »
Overexpression in transgenic tobacco reveals different roles for the rice homeodomain gene OsBIHD1 in biotic and abiotic stress responses.
H. Luo, F. Song, and Z. Zheng (2005)
J. Exp. Bot. 56, 2673-2682
   Abstract »    Full Text »    PDF »
Soluble methionine enhances accumulation of a 15 kDa zein, a methionine-rich storage protein, in transgenic alfalfa but not in transgenic tobacco plants.
G. Amira, M. Ifat, A. Tal, B. Hana, G. Shmuel, and A. Rachel (2005)
J. Exp. Bot. 56, 2443-2452
   Abstract »    Full Text »    PDF »
Co-expression of Cytochrome b 561 and Ascorbate Oxidase in Leaves of Wild Watermelon under Drought and High Light Conditions.
Y. Nanasato, K. Akashi, and A. Yokota (2005)
Plant Cell Physiol. 46, 1515-1524
   Abstract »    Full Text »    PDF »
Tetrahydrocannabinolic Acid Synthase, the Enzyme Controlling Marijuana Psychoactivity, is Secreted into the Storage Cavity of the Glandular Trichomes.
S. Sirikantaramas, F. Taura, Y. Tanaka, Y. Ishikawa, S. Morimoto, and Y. Shoyama (2005)
Plant Cell Physiol. 46, 1578-1582
   Abstract »    Full Text »    PDF »
Functional Conservation of PISTILLATA Activity in a Pea Homolog Lacking the PI Motif.
A. Berbel, C. Navarro, C. Ferrandiz, L. A. Canas, J.-P. Beltran, and F. Madueno (2005)
Plant Physiology 139, 174-185
   Abstract »    Full Text »    PDF »
NpPDR1, a Pleiotropic Drug Resistance-Type ATP-Binding Cassette Transporter from Nicotiana plumbaginifolia, Plays a Major Role in Plant Pathogen Defense.
Y. Stukkens, A. Bultreys, S. Grec, T. Trombik, D. Vanham, and M. Boutry (2005)
Plant Physiology 139, 341-352
   Abstract »    Full Text »    PDF »
Identification of an interactor of cadmium ion-induced glycine-rich protein involved in regulation of callose levels in plant vasculature.
S. Ueki and V. Citovsky (2005)
PNAS 102, 12089-12094
   Abstract »    Full Text »    PDF »
Homoglutathione Confers Tolerance to Acifluorfen in Transgenic Tobacco Plants Expressing Soybean Homoglutathione Synthetase.
A. Sugiyama and J. Sekiya (2005)
Plant Cell Physiol. 46, 1428-1432
   Abstract »    Full Text »    PDF »
Effects of Calreticulin on Viral Cell-to-Cell Movement.
M.-H. Chen, G.-W. Tian, Y. Gafni, and V. Citovsky (2005)
Plant Physiology 138, 1866-1876
   Abstract »    Full Text »    PDF »
Targeting the NAD7 Subunit to Mitochondria Restores a Functional Complex I and a Wild Type Phenotype in the Nicotiana sylvestris CMS II Mutant Lacking nad7.
B. Pineau, C. Mathieu, C. Gerard-Hirne, R. De Paepe, and P. Chetrit (2005)
J. Biol. Chem. 280, 25994-26001
   Abstract »    Full Text »    PDF »
Phage display-selected single-chain antibodies confer high levels of resistance against Tomato spotted wilt virus.
M. Prins, D. Lohuis, A. Schots, and R. Goldbach (2005)
J. Gen. Virol. 86, 2107-2113
   Abstract »    Full Text »    PDF »
A Functional Histidine-Tagged Replication Initiator Protein: Implications for the Study of Single-Stranded DNA Virus Replication In Planta.
J. C. Vega-Arreguin, T. Timchenko, B. Gronenborn, and B. C. Ramirez (2005)
J. Virol. 79, 8422-8430
   Abstract »    Full Text »    PDF »
Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco.
N. Shirasawa-Seo, Y. Sano, S. Nakamura, T. Murakami, S. Seo, Y. Ohashi, Y. Hashimoto, and T. Matsumoto (2005)
J. Gen. Virol. 86, 1851-1860
   Abstract »    Full Text »    PDF »
Phylloplanins of Tobacco Are Defensive Proteins Deployed on Aerial Surfaces by Short Glandular Trichomes.
R. W. Shepherd, W. T. Bass, R. L. Houtz, and G. J. Wagner (2005)
PLANT CELL 17, 1851-1861
   Abstract »    Full Text »    PDF »
A Peroxiredoxin Q Homolog from Gentians is Involved in Both Resistance Against Fungal Disease and Oxidative Stress.
A. Kiba, M. Nishihara, N. Tsukatani, T. Nakatsuka, Y. Kato, and S. Yamamura (2005)
Plant Cell Physiol. 46, 1007-1015
   Abstract »    Full Text »    PDF »
Increased Sedoheptulose-1,7-Bisphosphatase Activity in Transgenic Tobacco Plants Stimulates Photosynthesis and Growth from an Early Stage in Development.
S. Lefebvre, T. Lawson, M. Fryer, O. V. Zakhleniuk, J. C. Lloyd, and C. A. Raines (2005)
Plant Physiology 138, 451-460
   Abstract »    Full Text »    PDF »
The Nuclear Shuttle Protein of Tomato Leaf Curl New Delhi Virus Is a Pathogenicity Determinant.
M. Hussain, S. Mansoor, S. Iram, A. N. Fatima, and Y. Zafar (2005)
J. Virol. 79, 4434-4439
   Abstract »    Full Text »    PDF »
Cytoplasm and chloroplasts are not suitable subcellular locations for {beta}-zein accumulation in transgenic plants.
M. Bellucci, F. De Marchis, R. Mannucci, R. Bock, and S. Arcioni (2005)
J. Exp. Bot. 56, 1205-1212
   Abstract »    Full Text »    PDF »
A central role of Arabidopsis thaliana ovate family proteins in networking and subcellular localization of 3-aa loop extension homeodomain proteins.
J. Hackbusch, K. Richter, J. Muller, F. Salamini, and J. F. Uhrig (2005)
PNAS 102, 4908-4912
   Abstract »    Full Text »    PDF »
Analysis of tombusvirus revertants to identify essential amino acid residues within RNA-dependent RNA polymerase motifs.
K. Boonrod, S. Chotewutmontri, D. Galetzka, and G. Krczal (2005)
J. Gen. Virol. 86, 823-826
   Abstract »    Full Text »    PDF »
Development of three different cell types is associated with the activity of a specific MYB transcription factor in the ventral petal of Antirrhinum majus flowers.
M. Perez-Rodriguez, F. W. Jaffe, E. Butelli, B. J. Glover, and C. Martin (2005)
Development 132, 359-370
   Abstract »    Full Text »    PDF »
Pea DNA helicase 45 overexpression in tobacco confers high salinity tolerance without affecting yield.
N. Sanan-Mishra, X. H. Pham, S. K. Sopory, and N. Tuteja (2005)
PNAS 102, 509-514
   Abstract »    Full Text »    PDF »
A Green Fluorescent Protein Fusion to Actin-Binding Domain 2 of Arabidopsis Fimbrin Highlights New Features of a Dynamic Actin Cytoskeleton in Live Plant Cells.
M. B. Sheahan, C. J. Staiger, R. J. Rose, and D. W. McCurdy (2004)
Plant Physiology 136, 3968-3978
   Abstract »    Full Text »    PDF »
Biopharmaceuticals derived from genetically modified plants.
D.A. Goldstein and J.A. Thomas (2004)
QJM 97, 705-716
   Abstract »    Full Text »    PDF »
Dissection of Floral Pollination Syndromes in Petunia.
J. Stuurman, M. E. Hoballah, L. Broger, J. Moore, C. Basten, and C. Kuhlemeier (2004)
Genetics 168, 1585-1599
   Abstract »    Full Text »    PDF »
Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1.
E. J. H. Ross, J. M. Stone, C. G. Elowsky, R. Arredondo-Peter, R. V. Klucas, and G. Sarath (2004)
J. Exp. Bot. 55, 1721-1731
   Abstract »    Full Text »    PDF »
Direct Role of a Viroid RNA Motif in Mediating Directional RNA Trafficking across a Specific Cellular Boundary.
Y. Qi, T. Pelissier, A. Itaya, E. Hunt, M. Wassenegger, and B. Ding (2004)
PLANT CELL 16, 1741-1752
   Abstract »    Full Text »    PDF »
Extracellular Invertase Is an Essential Component of Cytokinin-Mediated Delay of Senescence.
M. E. Balibrea Lara, M.-C. Gonzalez Garcia, T. Fatima, R. Ehness, T. K. Lee, R. Proels, W. Tanner, and T. Roitsch (2004)
PLANT CELL 16, 1276-1287
   Abstract »    Full Text »    PDF »
cis-Regulatory Elements for Mesophyll-Specific Gene Expression in the C4 Plant Flaveria trinervia, the Promoter of the C4 Phosphoenolpyruvate Carboxylase Gene.
U. Gowik, J. Burscheidt, M. Akyildiz, U. Schlue, M. Koczor, M. Streubel, and P. Westhoff (2004)
PLANT CELL 16, 1077-1090
   Abstract »    Full Text »    PDF »
An N-terminal Peptide Extension Results in Efficient Expression, but not Secretion, of a Synthetic Horseradish Peroxidase Gene in Transgenic Tobacco.
M. KIS, E. BURBRIDGE, I. W. BROCK, L. HEGGIE, P. J. DIX, and T. A. KAVANAGH (2004)
Ann. Bot. 93, 303-310
   Abstract »    Full Text »    PDF »
GFP-labelled Rubisco and aspartate aminotransferase are present in plastid stromules and traffic between plastids.
E. Y. Kwok and M. R. Hanson (2004)
J. Exp. Bot. 55, 595-604
   Abstract »    Full Text »    PDF »
Expression of a Nondegradable Cyclin B1 Affects Plant Development and Leads to Endomitosis by Inhibiting the Formation of a Phragmoplast.
M. Weingartner, M.-C. Criqui, T. Meszaros, P. Binarova, A.-C. Schmit, A. Helfer, A. Derevier, M. Erhardt, L. Bogre, and P. Genschik (2004)
PLANT CELL 16, 643-657
   Abstract »    Full Text »    PDF »
NopL, an Effector Protein of Rhizobium sp. NGR234, Thwarts Activation of Plant Defense Reactions.
A. V. Bartsev, W. J. Deakin, N. M. Boukli, C. B. McAlvin, G. Stacey, P. Malnoe, W. J. Broughton, and C. Staehelin (2004)
Plant Physiology 134, 871-879
   Abstract »    Full Text »    PDF »
Plant MAPK Phosphatase Interacts with Calmodulins.
H. Yamakawa, S. Katou, S. Seo, I. Mitsuhara, H. Kamada, and Y. Ohashi (2004)
J. Biol. Chem. 279, 928-936
   Abstract »    Full Text »    PDF »
Engineering Plant Shikimate Pathway for Production of Tocotrienol and Improving Herbicide Resistance.
P. Rippert, C. Scimemi, M. Dubald, and M. Matringe (2004)
Plant Physiology 134, 92-100
   Abstract »    Full Text »    PDF »
Increased and Altered Fragrance of Tobacco Plants after Metabolic Engineering Using Three Monoterpene Synthases from Lemon.
J. Lucker, W. Schwab, B. van Hautum, J. Blaas, L. H. W. van der Plas, H. J. Bouwmeester, and H. A. Verhoeven (2004)
Plant Physiology 134, 510-519
   Abstract »    Full Text »    PDF »
Activation of the Fatty Acid {alpha}-Dioxygenase Pathway during Bacterial Infection of Tobacco Leaves: FORMATION OF OXYLIPINS PROTECTING AGAINST CELL DEATH.
M. Hamberg, A. Sanz, M. J. Rodriguez, A. P. Calvo, and C. Castresana (2003)
J. Biol. Chem. 278, 51796-51805
   Abstract »    Full Text »    PDF »
Genetic engineering of the glyoxalase pathway in tobacco leads to enhanced salinity tolerance.
S. L. Singla-Pareek, M. K. Reddy, and S. K. Sopory (2003)
PNAS 100, 14672-14677
   Abstract »    Full Text »    PDF »
Activation of Anthocyanin Biosynthesis in Gerbera hybrida (Asteraceae) Suggests Conserved Protein-Protein and Protein-Promoter Interactions between the Anciently Diverged Monocots and Eudicots.
P. Elomaa, A. Uimari, M. Mehto, V. A. Albert, R. A.E. Laitinen, and T. H. Teeri (2003)
Plant Physiology 133, 1831-1842
   Abstract »    Full Text »
Evidence from Engineering that Decarboxylation of Free Serine is the Major Source of Ethanolamine Moieties in Plants.
D. Rontein, D. Rhodes, and A. D. Hanson (2003)
Plant Cell Physiol. 44, 1185-1191
   Abstract »    Full Text »    PDF »
Polyamine Homeostasis in Transgenic Plants Overexpressing Ornithine Decarboxylase Includes Ornithine Limitation.
M. J. Mayer and A. J. Michael (2003)
J. Biochem. 134, 765-772
   Abstract »    Full Text »    PDF »
Translation Start Sequences Affect the Efficiency of Silencing of Agrobacterium tumefaciens T-DNA Oncogenes.
H. Lee, J. L. Humann, J. S. Pitrak, J. T. Cuperus, T. D. Parks, C. A. Whistler, M. C. Mok, and L. W. Ream (2003)
Plant Physiology 133, 966-977
   Abstract »    Full Text »    PDF »
Site-Specific Integration of Agrobacterium tumefaciens T-DNA via Double-Stranded Intermediates.
T. Tzfira, L. R. Frankman, M. Vaidya, and V. Citovsky (2003)
Plant Physiology 133, 1011-1023
   Abstract »    Full Text »    PDF »
Production of FaeG, the Major Subunit of K88 Fimbriae, in Transgenic Tobacco Plants and Its Immunogenicity in Mice.
Y. Huang, W. Liang, A. Pan, Z. Zhou, C. Huang, J. Chen, and D. Zhang (2003)
Infect. Immun. 71, 5436-5439
   Abstract »    Full Text »    PDF »
Ectopic Expression of an Orchid (Oncidium Gower Ramsey) AGL6-like Gene Promotes Flowering by Activating Flowering Time Genes in Arabidopsis thaliana.
H.-F. Hsu, C.-H. Huang, L.-T. Chou, and C.-H. Yang (2003)
Plant Cell Physiol. 44, 783-794