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Science 8 February 1985:
Vol. 227. no. 4687, pp. 645 - 646
DOI: 10.1126/science.3969554
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Articles

Science, Vol 227, Issue 4687, 645-646
Copyright © 1985 by American Association for the Advancement of Science

articles

A single recombinant plasmid expressing two major outer surface proteins of the Lyme disease spirochete

TR Howe, LW Mayer, and AG Barbour

A gene bank of DNA from the Lyme disease spirochete was constructed in the plasmid pBR322. Plasmid pTRH32, a recombinant that in Escherichia coli expresses the two major outer surface proteins of the Lyme disease spirochete, was identified. One of the recombinant products, designated OspA, represents a surface protein that appears to be common to all Lyme disease spirochetes, whereas the other recombinant product, designated OspB, represents a more variable surface protein. This recombinant plasmid provides a foundation for future studies on the epidemiology and pathogenesis of Lyme disease as well as on the genetic organization of the etiologic agent.


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A Genome-Wide Proteome Array Reveals a Limited Set of Immunogens in Natural Infections of Humans and White-Footed Mice with Borrelia burgdorferi.
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Crystal Structure of Neurotropism-Associated Variable Surface Protein 1 (Vsp1) of Borrelia turicatae..
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Lipoprotein computational prediction in spirochaetal genomes.
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Immunoglobulin-Regulated Expression of Borrelia burgdorferi Outer Surface Protein A In Vivo.
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Structural Investigation of Borrelia burgdorferi OspB, a BactericidalFab Target.
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An ecological approach to preventing human infection: Vaccinating wild mouse reservoirs intervenes in the Lyme disease cycle.
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PNAS 101, 18159-18164
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Borrelia Outer Membrane Surface Proteins and Transmission Through the Tick.
T. J. Templeton (2004)
J. Exp. Med. 199, 603-606
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Host-Adapted Borrelia burgdorferi in Mice Expresses OspA during Inflammation.
H. Crowley and B. T. Huber (2003)
Infect. Immun. 71, 4003-4010
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Access of Antibody or Trypsin to an Integral Outer Membrane Protein (P66) of Borrelia burgdorferi Is Hindered by Osp Lipoproteins.
J. Bunikis and A. G. Barbour (1999)
Infect. Immun. 67, 2874-2883
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The Immunoglobulin (IgG) Antibody Response to OspA and OspB Correlates with Severe and Prolonged Lyme Arthritis and the IgG Response to P35 Correlates with Mild and Brief Arthritis.
E. Akin, G. L. McHugh, R. A. Flavell, E. Fikrig, and A. C. Steere (1999)
Infect. Immun. 67, 173-181
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Lyme Disease Borrelia Species in Northeastern China Resemble Those Isolated from Far Eastern Russia and Japan.
M. Li, T. Masuzawa, N. Takada, F. Ishiguro, H. Fujita, A. Iwaki, H. Wang, J. Wang, M. Kawabata, and Y. Yanagihara (1998)
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Active and Passive Immunity against Borrelia burgdorferi Decorin Binding Protein A (DbpA) Protects against Infection.
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Infect. Immun. 66, 2143-2153
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Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab.
H. Li, J. J. Dunn, B. J. Luft, and C. L. Lawson (1997)
PNAS 94, 3584-3589
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The biological and social phenomenon of Lyme disease.
A. Barbour and D Fish (1993)
Science 260, 1610-1616
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Positive Lyme Serology in Subacute Bacterial Endocarditis: A Study of Four Patients.
A. T. Kaell, D. J. Volkman, P. D. Gorevic, and R. J. Dattwyler (1990)
JAMA 264, 2916-2918
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Linear plasmids of the bacterium Borrelia burgdorferi have covalently closed ends.
A. Barbour and C. Garon (1987)
Science 237, 409-411
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Cloned gene of Rickettsia rickettsii surface antigen: candidate vaccine for Rocky Mountain spotted fever.
G. McDonald, R. Anacker, and K Garjian (1987)
Science 235, 83-85
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Structural Conservation of Neurotropism-associated VspA within the Variable Borrelia Vsp-OspC Lipoprotein Family.
W. R. Zuckert, T. A. Kerentseva, C. L. Lawson, and A. G. Barbour (2001)
J. Biol. Chem. 276, 457-463
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