Science, Vol 226, Issue 4679, 1211-1213
Copyright © 1984 by American Association for the Advancement of Science
Cloned mycoplasma ribosomal RNA genes for the detection of mycoplasma contamination in tissue cultures
UB Gobel
and
EJ Stanbridge
A cloned fragment of the mycoplasma ribosomal RNA operon was used as a molecular probe for the detection of mycoplasmas in cell cultures. According to the conditions of hybridization, the probe can detect prokaryotes in general or mycoplasmas specifically.