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Science 9 November 1984:
Vol. 226. no. 4675, pp. 696 - 698
DOI: 10.1126/science.6387912
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Articles

Science, Vol 226, Issue 4675, 696-698
Copyright © 1984 by American Association for the Advancement of Science

articles

Molecular weight determinations of proteins by californium plasma desorption mass spectrometry

B Sundqvist, P Roepstorff, J Fohlman, A Hedin, P Hakansson, I Kamensky, M Lindberg, M Salehpour, and G Sawe

The plasma desorption mass spectrometry method is used to determine the molecular weights of larger molecules than before, to determine the molecular weights of proteins and peptides in mixtures, and to monitor protein modification reactions. Proteins up to molecular weight 25,000 can now be studied with a mass spectrometric technique. Protein-peptide mixtures that could not be resolved with conventional techniques were successfully analyzed by this technique. The precision of the method is good enough to permit one to follow the different steps in the conversion of porcine insulin to human insulin.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Primary sequence information from intact proteins by electrospray ionization tandem mass spectrometry.
J. Loo, C. Edmonds, and R. Smith (1990)
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Characterization by tandem mass spectrometry of structural modifications in proteins.
K Biemann and H. Scoble (1987)
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