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Science 19 October 1984:
Vol. 226. no. 4672, pp. 304 - 311
DOI: 10.1126/science.6385254
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Articles

Science, Vol 226, Issue 4672, 304-311
Copyright © 1984 by American Association for the Advancement of Science

articles

Contemporary methodology for protein structure determination

MW Hunkapiller, JE Strickler, and KJ Wilson

The techniques used for the characterization of protein and peptide structure have undergone great changes that have improved the speed, reliability, and applicability of the process. High-performance liquid chromatography and gel electrophoresis have made the purification of proteins and peptides a routine procedure, even when the compound of interest is a minor component of a complex biological mixture. The chemistry and instrumentation used in amino acid analysis and amino acid sequencing now permit the analysis of as little as 5 to 50 picomoles of samples. This represents an increase in sensitivity of more than a thousandfold over the last 10 years and has made possible the structural analysis of a wide variety of scarce but important compounds.


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