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Science 31 August 1984:
Vol. 225. no. 4665, pp. 935 - 938
DOI: 10.1126/science.6089334
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Articles

Science, Vol 225, Issue 4665, 935-938
Copyright © 1984 by American Association for the Advancement of Science

articles

Small DNA deletions creating avirulence in Streptococcus pyogenes

JG Spanier, SJ Jones, and P Cleary

The M protein is the antigen on the surface of group A streptococci that allows these bacteria to resist phagocytosis. DNA encoding the M12 protein was cloned into Escherichia coli and used as an isotopically labeled hybridization probe to compare genomic DNA's isolated from M+ and M- isogenic cultures in an effort to elucidate the genetic basis of this variation. DNA's from two spontaneous, independent M- variants contained small (approximately 50 base pairs) deletions which were mapped to identical restriction fragments within or adjacent to the M protein coding sequence. Taken together with the pleiotropic nature of these deletions, this suggests that they define a regulatory switch.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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A. H. Nobbs, R. J. Lamont, and H. F. Jenkinson (2009)
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Pathogenesis of Group A Streptococcal Infections.
M. W. Cunningham (2000)
Clin. Microbiol. Rev. 13, 470-511
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