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Science 31 August 1984:
Vol. 225. no. 4665, pp. 932 - 935
DOI: 10.1126/science.6382607
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Articles

Science, Vol 225, Issue 4665, 932-935
Copyright © 1984 by American Association for the Advancement of Science

articles

Heparin binds endothelial cell growth factor, the principal endothelial cell mitogen in bovine brain

T Maciag, T Mehlman, R Friesel, and AB Schreiber

Endothelial cell growth factor (ECGF), an anionic polypeptide mitogen, binds to immobilized heparin. The interaction between the acidic polypeptide and the anionic carbohydrate suggests a mechanism that is independent of ion exchange. Monoclonal antibodies to purified bovine ECGF inhibited the biological activity of ECGF in crude preparations of bovine brain. These data indicate that ECGF is the principal mitogen for endothelial cells from bovine brain, that heparin affinity chromatography may be used to purify and concentrate ECGF, and that the affinity of ECGF for heparin may have structural and perhaps biological significance.


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