Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 31 August 1984:
Vol. 225. no. 4665, pp. 898 - 903
DOI: 10.1126/science.6206566
Prev | Table of Contents | Next

Articles

Science, Vol 225, Issue 4665, 898-903
Copyright © 1984 by American Association for the Advancement of Science

articles

Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors

RA Padgett, MM Konarska, PJ Grabowski, SF Hardy, and PA Sharp

The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5' end of the intervening sequence joined to a site near the 3' splice site. This lariat structure, which has been characterized for an adenovirus 2 major late transcript, has a branch point, with 2'-5' and 3'-5' phosphodiester bonds emanating from a single adenosine residue. The excised intervening sequence retains the branch site and terminates in a guanosine residue with a 3' hydroxyl group. The phosphate group at the splice junction between the two exons originates from the 3' splice site at the precursor.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification and characterization of a short 2'-3' bond-forming ribozyme.
D. J. Smith and M. M. Konarska (2009)
RNA 15, 8-13
   Abstract »    Full Text »    PDF »
Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.
S. Valadkhan, A. Mohammadi, C. Wachtel, and J. L. Manley (2007)
RNA 13, 2300-2311
   Abstract »    Full Text »    PDF »
Structure-function analysis of yeast RNA debranching enzyme (Dbr1), a manganese-dependent phosphodiesterase.
M. F. Khalid, M. J. Damha, S. Shuman, and B. Schwer (2005)
Nucleic Acids Res. 33, 6349-6360
   Abstract »    Full Text »    PDF »
An evaluation of detection methods for large lariat RNAs.
C. E. COOMBES and J. D. BOEKE (2005)
RNA 11, 323-331
   Abstract »    Full Text »    PDF »
Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase.
M. Mendel-Hartvig, A. Kumar, and U. Landegren (2004)
Nucleic Acids Res. 32, e2
   Abstract »    Full Text »    PDF »
Effects of RNase L Mutations Associated with Prostate Cancer on Apoptosis Induced by 2',5'-Oligoadenylates.
Y. Xiang, Z. Wang, J. Murakami, S. Plummer, E. A. Klein, J. D. Carpten, J. M. Trent, W. B. Isaacs, G. Casey, and R. H. Silverman (2003)
Cancer Res. 63, 6795-6801
   Abstract »    Full Text »    PDF »
Primer on Medical Genomics Part II: Background Principles and Methods in Molecular Genetics.
A. Tefferi, E. D. Wieben, G. W. Dewald, D. A. H. Whiteman, M. E. Bernard, and T. C. Spelsberg (2002)
Mayo Clin. Proc. 77, 785-808
   Abstract »    PDF »
Characterization of the major laccase isoenzyme from Trametes pubescens and regulation of its synthesis by metal ions.
C. Galhaup, S. Goller, C. K. Peterbauer, J. Strauss, and D. Haltrich (2002)
Microbiology 148, 2159-2169
   Abstract »    Full Text »    PDF »
Herpes Simplex Virus IE63 (ICP27) Protein Interacts with Spliceosome-Associated Protein 145 and Inhibits Splicing prior to the First Catalytic Step.
H. E. Bryant, S. E. Wadd, A. I. Lamond, S. J. Silverstein, and J. B. Clements (2001)
J. Virol. 75, 4376-4385
   Abstract »    Full Text »    PDF »
Alternative pre-mRNA splicing of the sterol 27-hydroxylase gene (CYP 27) caused by a G to A mutation at the last nucleotide of exon 6 in a patient with cerebrotendinous xanthomatosis (CTX).
W. Chen, S. Kubota, and Y. Seyama (1998)
J. Lipid Res. 39, 509-517
   Abstract »    Full Text »
Circular Ribozymes Generated in Escherichia coli Using Group I Self-splicing Permuted Intron-Exon Sequences.
M. Puttaraju and M. D. Been (1996)
J. Biol. Chem. 271, 26081-26087
   Abstract »    Full Text »    PDF »
Association of U6 snRNA with the 5'-splice site region of pre-mRNA in the spliceosome..
H Sawa and Y Shimura (1992)
Genes & Dev. 6, 244-254
   Abstract »    PDF »
Modulation of alternative splicing of adenoviral E1A transcripts: factors involved in the early-to-late transition..
R Gattoni, K Chebli, M Himmelspach, and J Stevenin (1991)
Genes & Dev. 5, 1847-1858
   Abstract »    PDF »
Unexpected point mutations activate cryptic 3' splice sites by perturbing a natural secondary structure within a yeast intron..
J O Deshler and J J Rossi (1991)
Genes & Dev. 5, 1252-1263
   Abstract »    PDF »
Identification of two distinct intron elements involved in alternative splicing of beta-tropomyosin pre-mRNA..
D M Helfman, R F Roscigno, G J Mulligan, L A Finn, and K S Weber (1990)
Genes & Dev. 4, 98-110
   Abstract »    PDF »
Spliceosome assembly in yeast..
S C Cheng and J Abelson (1987)
Genes & Dev. 1, 1014-1027
   Abstract »    PDF »
A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation..
J R Couto, J Tamm, R Parker, and C Guthrie (1987)
Genes & Dev. 1, 445-455
   Abstract »    PDF »
A novel role for the 3' region of introns in pre-mRNA splicing of Saccharomyces cerevisiae..
B C Rymond, D D Torrey, and M Rosbash (1987)
Genes & Dev. 1, 238-246
   Abstract »    PDF »
Splicing of messenger RNA precursors.
P. Sharp (1987)
Science 235, 766-771
   Abstract »    PDF »
Structures Involved in Tetrahymena rRNA Self-splicing and RNA Enzyme Activity.
M.D. Been, E.T. Barfod, J.M. Burke, J.V. Price, N.K. Tanner, A.J. Zaug, and T.R. Cech (1987)
Cold Spring Harb Symp Quant Biol 52, 147-157
   Abstract »    PDF »
Group II Intron Self-splicing: Development of Alternative Reaction Conditions and Identification of a Predicted Intermediate.
C.L. Peebles, E.J. Benatan, K.A. Jarrell, and P.S. Perlman (1987)
Cold Spring Harb Symp Quant Biol 52, 223-232
   Abstract »    PDF »
Splicing of Messenger RNA Precursors.
P.A. Sharp, M.M. Konarksa, P.J. Grabowski, A.I. Lamond, R. Marciniak, and S.R. Seiler (1987)
Cold Spring Harb Symp Quant Biol 52, 277-285
   Abstract »    PDF »
Affinity chromatography of splicing complexes: U2, U5, and U4 + U6 small nuclear ribonucleoprotein particles in the spliceosome.
P. Grabowski and P. Sharp (1986)
Science 233, 1294-1299
   Abstract »    PDF »
Heterogeneous nuclear ribonucleoproteins: role in RNA splicing.
Y. Choi, P. Grabowski, P. Sharp, and G Dreyfuss (1986)
Science 231, 1534-1539
   Abstract »    PDF »
The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
B Chabot, D. Black, D. LeMaster, and J. Steitz (1985)
Science 230, 1344-1349
   Abstract »    PDF »
An RNA processing activity that debranches RNA lariats.
B Ruskin and M. Green (1985)
Science 229, 135-140
   Abstract »    PDF »
The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction.
E Brody and J Abelson (1985)
Science 228, 963-967
   Abstract »    PDF »
Mutational Analysis of 3' Splice Site Selection during trans-Splicing.
H. S. Hummel, R. D. Gillespie, and J. Swindle (2000)
J. Biol. Chem. 275, 35522-35531
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)