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Science 27 July 1984:
Vol. 225. no. 4660, pp. 434 - 437
DOI: 10.1126/science.6377495
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Articles

Science, Vol 225, Issue 4660, 434-437
Copyright © 1984 by American Association for the Advancement of Science

articles

Sedimentation field flow fractionation of DNA's

LE Schallinger, WW Yau, and JJ Kirkland

Sedimentation field flow fractionation (SFFF) is a method for purifying and providing mass or size distribution information on samples containing particulates or soluble macromolecules. Since SFFF separations are based on simple physical phenomena related to first principles, molecular weight (or particle sizes) can be determined without calibration standards. SFFF is a gentle technique suited for fractionating biomolecules. Studies with the fragile lambda DNA (molecular weight, 33 X 10(6] and smaller supercoiled plasmids have shown that these materials are not altered during SFFF separation; molecular weights and conformation remain unchanged, and biological activity is not reduced. Recoveries of nucleic acids approach 100 percent. Typically, components with about 20 percent difference in mass can be separated essentially to baseline if required. Fractionation time is usually independent of molecular weight, and separations often can be carried out within an hour.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Field-flow fractionation: analysis of macromolecular, colloidal, and particulate materials.
J. Giddings (1993)
Science 260, 1456-1465
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