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Science 11 May 1984:
Vol. 224. no. 4649, pp. 603 - 605
DOI: 10.1126/science.6710159
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Articles

Science, Vol 224, Issue 4649, 603-605
Copyright © 1984 by American Association for the Advancement of Science

articles

Complete development of Cryptosporidium in cell culture

WL Current and TB Haynes

Protozoan parasites of the genus Cryptosporidium cause a short-term, flu-like, gastrointestinal illness in immunocompetent persons and severe, persistent, life-threatening diarrhea in immunodeficient individuals. No effective therapy is available for the treatment of cryptosporidiosis in the immunodeficient host. Complete development (from sporozoite to sporulated oocyst) of a human isolate of Cryptosporidium was achieved in cultured human fetal lung cells and primary chicken kidney and porcine kidney cells. The growth of this newly recognized zoonotic agent in cell culture now provides a means of studying its behavior, development, and metabolism, and a mechanism for evaluation of potentially useful therapeutic agents.


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Comparison of In Vitro Cell Culture and a Mouse Assay for Measuring Infectivity of Cryptosporidium parvum.
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In Vitro Cultivation of Cryptosporidium Species.
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Decrease in Cryptosporidium parvum Oocyst Infectivity In Vitro by Using the Membrane Filter Dissolution Method for Recovering Oocysts from Water Samples.
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