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Science 4 May 1984:
Vol. 224. no. 4648, pp. 503 - 505
DOI: 10.1126/science.6200937
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Articles

Science, Vol 224, Issue 4648, 503-505
Copyright © 1984 by American Association for the Advancement of Science

articles

Serological analysis of a subgroup of human T-lymphotropic retroviruses (HTLV-III) associated with AIDS

J Schupbach, M Popovic, RV Gilden, MA Gonda, MG Sarngadharan, and RC Gallo

The two main subgroups of the family of human T-lymphotropic retroviruses (HTLV) that have previously been characterized are known as HTLV-I and HTLV-II. Both are associated with certain human leukemias and lymphomas. Cell surface antigens (p61 and p65) encoded by HTLV-I are frequently recognized, at low titers, by antibodies in the serum of patients with acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that precede AIDS (pre-AIDS). This suggests an involvement of HTLV in these disorders. Another subgroup of HTLV, designated HTLV-III, has now been isolated from many patients with AIDS and pre-AIDS. In the studies described in this report, virus-associated antigens in T-cell clones permanently producing HTLV-III were subjected to biochemical and immunological analyses. Antigens of HTLV-III, specifically detected by antibodies in serum from AIDS or pre-AIDS patients and revealed by the Western blot technique, are similar in size to those found in other subgroups of HTLV. They include at least three serologically unrelated antigenic groups, one of which is associated with group-specific antigens (p55 and P24) and another with envelope-related (p65) proteins, while the antigens in the third group are of unknown affiliation. The data show that HTLV-III is clearly distinguishable from HTLV-I and HTLV-II but is also significantly related to both viruses. HTLV-III is thus a true member of the HTLV family.


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   Abstract »    PDF »
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Characterization of long terminal repeat sequences of HTLV-III.
B Starcich, L Ratner, S. Josephs, T Okamoto, R. Gallo, and F Wong-Staal (1985)
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   Abstract »    PDF »
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G. B. Scott, M. A. Fischl, N. Klimas, M. A. Fletcher, G. M. Dickinson, R. S. Levine, and W. P. Parks (1985)
JAMA 253, 363-366
   Abstract »    PDF »
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S. H. Weiss, J. J. Goedert, M. G. Sarngadharan, A. J. Bodner, The AIDS Seroepidemiology Collaborative Working Gr, R. C. Gallo, and W. A. Blattner (1985)
JAMA 253, 221-225
   Abstract »    PDF »
Trans-acting transcriptional regulation of human T-cell leukemia virus type III long terminal repeat.
J Sodroski, C Rosen, F Wong-Staal, S. Salahuddin, M Popovic, S Arya, R. Gallo, and W. Haseltine (1985)
Science 227, 171-173
   Abstract »    PDF »
Sequence homology and morphologic similarity of HTLV-III and visna virus, a pathogenic lentivirus.
M. Gonda, F Wong-Staal, R. Gallo, J. Clements, O Narayan, and R. Gilden (1985)
Science 227, 173-177
   Abstract »    PDF »
HTLV-III infection in brains of children and adults with AIDS encephalopathy.
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Science 227, 177-182
   Abstract »    PDF »
Apparent Transmission of Human T-Cell Leukemia Virus Type III to a Heterosexual Woman with the Acquired Immunodeficiency Syndrome.
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Ann Intern Med 102, 63-66
   Abstract »    PDF »
Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome.
G. Shaw, B. Hahn, S. Arya, J. Groopman, R. Gallo, and F Wong-Staal (1984)
Science 226, 1165-1171
   Abstract »    PDF »
Whither Immunization Against Viral Infections?.
M. R. HILLEMAN (1984)
Ann Intern Med 101, 852-858
   Abstract »    PDF »
HTLV-III in saliva of people with AIDS-related complex and healthy homosexual men at risk for AIDS.
J. Groopman, S. Salahuddin, M. Sarngadharan, P. Markham, M Gonda, A Sliski, and R. Gallo (1984)
Science 226, 447-449
   Abstract »    PDF »
HTLV-III in cells cultured from semen of two patients with AIDS.
D Zagury, J Bernard, J Leibowitch, B Safai, J. Groopman, M Feldman, M. Sarngadharan, and R. Gallo (1984)
Science 226, 449-451
   Abstract »    PDF »
The Acquired Immunodeficiency Syndrome: Commentary.
Council on Scientific Affairs (1984)
JAMA 252, 2037-2043
   Abstract »    PDF »
Suramin protection of T cells in vitro against infectivity and cytopathic effect of HTLV-III.
H Mitsuya, M Popovic, R Yarchoan, S Matsushita, R. Gallo, and S Broder (1984)
Science 226, 172-174
   Abstract »    PDF »
Antigens encoded by the 3'-terminal region of human T-cell leukemia virus: evidence for a functional gene.
T. Lee, J. Coligan, J. Sodroski, W. Haseltine, S. Salahuddin, F Wong-Staal, R. Gallo, and M Essex (1984)
Science 226, 57-61
   Abstract »    PDF »
Functional properties of antigen-specific T cells infected by human T-cell leukemia-lymphoma virus (HTLV-I).
H Mitsuya, H. Guo, J Cossman, M Megson, M. Reitz Jr, and S Broder (1984)
Science 225, 1484-1486
   Abstract »    PDF »
Etiology of AIDS.
A. J. Ammann (1984)
JAMA 252, 1281-1282
   Abstract »    PDF »
Homology of genome of AIDS-associated virus with genomes of human T-cell leukemia viruses.
S. Arya, R. Gallo, B. Hahn, G. Shaw, M Popovic, S. Salahuddin, and F Wong-Staal (1984)
Science 225, 927-930
   Abstract »    PDF »
Structure of 3' terminal region of type II human T lymphotropic virus: evidence for new coding region.
W. Haseltine, J Sodroski, R Patarca, D Briggs, D Perkins, and F Wong-Staal (1984)
Science 225, 419-421
   Abstract »    PDF »
Sequence of the envelope glycoprotein gene of type II human T lymphotropic virus.
J Sodroski, R Patarca, D Perkins, D Briggs, T. Lee, M Essex, J Coligan, F Wong-Staal, R. Gallo, and W. Haseltine (1984)
Science 225, 421-424
   Abstract »    PDF »
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V. Kalyanaraman, C. Cabradilla, J. Getchell, R Narayanan, E. Braff, J. Chermann, F Barre-Sinoussi, L Montagnier, T. Spira, J Kaplan, et al. (1984)
Science 225, 321-323
   Abstract »    PDF »
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M Popovic, M. Sarngadharan, E Read, and R. Gallo (1984)
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   Abstract »    PDF »
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R. Gallo, S. Salahuddin, M Popovic, G. Shearer, M Kaplan, B. Haynes, T. Palker, R Redfield, J Oleske, B Safai, et al. (1984)
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   Abstract »    PDF »
Antibodies reactive with human T-lymphotropic retroviruses (HTLV-III) in the serum of patients with AIDS.
M. Sarngadharan, M Popovic, L Bruch, J Schupbach, and R. Gallo (1984)
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