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Science 20 January 1984:
Vol. 223. no. 4633, pp. 289 - 291
DOI: 10.1126/science.6422551
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Articles

Science, Vol 223, Issue 4633, 289-291
Copyright © 1984 by American Association for the Advancement of Science

articles

Fluorescence-line-narrowed spectra of polycyclic aromatic carcinogen-DNA adducts

V Heisig, AM Jeffrey, MJ McGlade, and GJ Small

The laser excited fluorescence-line-narrowed spectrum of DNA modified with (+/-)-r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), the ultimate carcinogenic metabolite of benzo[a]pyrene (BP), has been obtained in a water-glycerol-ethanol glass at 4.2 K. The spectrum was well resolved and highly characteristic of the chromophore. Comparisons were made between the spectrum of this modified DNA and the isolated deoxyguanosine-BPDE adduct and a series of other 7,8,9,10-tetrahydro-BP (THBP) derivatives. 9-Hydroxy-BP 4,5-oxide, which is also involved in the binding of BP to DNA, and THBP have very similar conventional broadband fluorescence spectra. However, the fluorescence-line-narrowed spectra of their derivatives were readily distinguishable either as individual components or as mixtures.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cross-reactivity and conformational multiplicity of an anti-polycyclic aromatic hydrocarbon mAb.
N. M. Grubor, J. Hayes, G. J. Small, and R. Jankowiak (2005)
PNAS 102, 7453-7458
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