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Science 23 December 1983:
Vol. 222. no. 4630, pp. 1339 - 1341
DOI: 10.1126/science.6689218
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Articles

Science, Vol 222, Issue 4630, 1339-1341
Copyright © 1983 by American Association for the Advancement of Science

articles

Biotinated probe containing a long-terminal repeat hybridized to a mouse colon tumor and normal tissue

ME Royston and LH Augenlicht

The cloned complementary DNA pMCT-1, which contains an intracisternal A particle long-terminal repeat, is more highly expressed in a mouse colon tumor than in the normal mouse colon. In situ hybridization of biotin-substituted pMCT-1 to fixed frozen sections shows that expression of pMCT-1 is seen throughout the tumor and is highly heterogeneous on a cellular basis, while expression is undetectable in any cell in the normal colonic mucosa.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)