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Science 18 November 1983:
Vol. 222. no. 4625, pp. 778 - 782
DOI: 10.1126/science.6356359
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Articles

Science, Vol 222, Issue 4625, 778-782
Copyright © 1983 by American Association for the Advancement of Science

articles

Yeast RNA polymerase II genes: isolation with antibody probes

RA Young and RW Davis

Genes encoding yeast RNA polymerase II subunits were cloned. Efficient isolation of these genes was accomplished by probing a phage lambda gt11 recombinant DNA expression library with polyvalent antibodies directed against purified yeast RNA polymerase II. The identity of genes that specify the largest RNA polymerase II subunits, the 220,000- and 150,000-dalton polypeptides, was confirmed by competitive radioimmune assay. Both of these genes exist in single copy in the yeast Saccharomyces cerevisiae.


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