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Science 21 October 1983:
Vol. 222. no. 4621, pp. 245 - 252
DOI: 10.1126/science.6353575
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Articles

Science, Vol 222, Issue 4621, 245-252
Copyright © 1983 by American Association for the Advancement of Science

articles

High-performance liquid chromatography of biopolymers

FE Regnier

The ability to separate biological macromolecules with good resolution on liquid chromatographic columns has depended on the development of suitable packing materials. In size exclusion chromatography, molecules are separated by size on the basis of differential permeation of the packing. Ion exchange, hydrophobic interaction (or reversed-phase), and affinity chromatography are all surface-mediated separation methods, although they depend on different retention mechanisms. High-performance liquid chromatographic columns designed for biopolymers offer major advantages over conventional columns in both speed and resolving power. The exponential growth of literature on the high-performance separation of peptides and proteins in particular indicates that the technique will become the dominant form of column liquid chromatography.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Surface Tensions of Proteins in Aqueous Solutions.
R. Z. Greenley (1987)
Journal of Bioactive and Compatible Polymers 2, 293-304
   Abstract »    PDF »
Trends in Analytical Scale Separations.
J. W. Jorgenson (1984)
Science 226, 254-261
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)