Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 14 October 1983:
Vol. 222. no. 4620, pp. 167 - 169
DOI: 10.1126/science.6353574
Prev | Table of Contents | Next

Articles

Science, Vol 222, Issue 4620, 167-169
Copyright © 1983 by American Association for the Advancement of Science

articles

Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesis of indigo

BD Ensley, BJ Ratzkin, TD Osslund, MJ Simon, LP Wackett, and DT Gibson

A fragment of plasmid NAH7 from Pseudomonas putida PpG7 has been cloned and expressed in Escherichia coli HB101. Growth of the recombinant Escherichia coli in nutrient medium results in the formation of indigo. The production of this dye is increased in the presence of tryptophan or indole. Several bacteria that oxidize aromatic hydrocarbons to cis-dihydrodiols also oxidize indole to indigo. The results suggest that indigo formation is due to the combined activities of tryptophanase and naphthalene dioxygenase.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mutagenic Activity of Indigofera truxillensis and I. suffruticosa Aerial Parts.
T. R. Calvo, C. R. P. Cardoso, A. C. da Silva Moura, L. C. dos Santos, I. M. S. Colus, W. Vilegas, and E. A. Varanda (2009)
Evid. Based Complement. Altern. Med.
   Abstract »    Full Text »    PDF »
Characterization of a Polycyclic Aromatic Hydrocarbon Degradation Gene Cluster in a Phenanthrene-Degrading Acidovorax Strain.
D. R. Singleton, L. Guzman Ramirez, and M. D. Aitken (2009)
Appl. Envir. Microbiol. 75, 2613-2620
   Abstract »    Full Text »    PDF »
Discovery of a Novel Styrene Monooxygenase Originating from the Metagenome.
E. W. van Hellemond, D. B. Janssen, and M. W. Fraaije (2007)
Appl. Envir. Microbiol. 73, 5832-5839
   Abstract »    Full Text »    PDF »
Enterohemorrhagic Escherichia coli Biofilms Are Inhibited by 7-Hydroxyindole and Stimulated by Isatin.
J. Lee, T. Bansal, A. Jayaraman, W. E. Bentley, and T. K. Wood (2007)
Appl. Envir. Microbiol. 73, 4100-4109
   Abstract »    Full Text »    PDF »
Mutations of Toluene-4-Monooxygenase That Alter Regiospecificity of Indole Oxidation and Lead to Production of Novel Indigoid Pigments.
K. McClay, C. Boss, I. Keresztes, and R. J. Steffan (2005)
Appl. Envir. Microbiol. 71, 5476-5483
   Abstract »    Full Text »    PDF »
Saturation Mutagenesis of Burkholderia cepacia R34 2,4-Dinitrotoluene Dioxygenase at DntAc Valine 350 for Synthesizing Nitrohydroquinone, Methylhydroquinone, and Methoxyhydroquinone.
B. G. Keenan, T. Leungsakul, B. F. Smets, and T. K. Wood (2004)
Appl. Envir. Microbiol. 70, 3222-3231
   Abstract »    Full Text »    PDF »
Saturation Mutagenesis of Toluene ortho-Monooxygenase of Burkholderia cepacia G4 for Enhanced 1-Naphthol Synthesis and Chloroform Degradation.
L. Rui, Y. M. Kwon, A. Fishman, K. F. Reardon, and T. K. Wood (2004)
Appl. Envir. Microbiol. 70, 3246-3252
   Abstract »    Full Text »    PDF »
Molecular Characterization and Substrate Preference of a Polycyclic Aromatic Hydrocarbon Dioxygenase from Cycloclasticus sp. Strain A5.
Y. Kasai, K. Shindo, S. Harayama, and N. Misawa (2003)
Appl. Envir. Microbiol. 69, 6688-6697
   Abstract »    Full Text »    PDF »
3- and 4-alkylphenol degradation pathway in Pseudomonas sp. strain KL28: genetic organization of the lap gene cluster and substrate specificities of phenol hydroxylase and catechol 2,3-dioxygenase.
J. J. Jeong, J. H. Kim, C.-K. Kim, I. Hwang, and K. Lee (2003)
Microbiology 149, 3265-3277
   Abstract »    Full Text »    PDF »
Degradation of Anthracene by Mycobacterium sp. Strain LB501T Proceeds via a Novel Pathway, through o-Phthalic Acid.
R. van Herwijnen, D. Springael, P. Slot, H. A. J. Govers, and J. R. Parsons (2003)
Appl. Envir. Microbiol. 69, 186-190
   Abstract »    Full Text »    PDF »
Flow Cytometry Analysis of Changes in the DNA Content of the Polychlorinated Biphenyl Degrader Comamonas testosteroni TK102: Effect of Metabolites on Cell-Cell Separation.
Y. Hiraoka, T. Yamada, K. Tone, Y. Futaesaku, and K. Kimbara (2002)
Appl. Envir. Microbiol. 68, 5104-5112
   Abstract »    Full Text »    PDF »
Hydroxylation of Indole by Laboratory-evolved 2-Hydroxybiphenyl 3-Monooxygenase.
A. Meyer, M. Wursten, A. Schmid, H.-P. E. Kohler, and B. Witholt (2002)
J. Biol. Chem. 277, 34161-34167
   Abstract »    Full Text »    PDF »
Monocyclic Aromatic Hydrocarbon Degradation by Rhodococcus sp. Strain DK17.
D. Kim, Y.-S. Kim, S.-K. Kim, S. W. Kim, G. J. Zylstra, Y. M. Kim, and E. Kim (2002)
Appl. Envir. Microbiol. 68, 3270-3278
   Abstract »    Full Text »    PDF »
Ion Regulation in the Brain: Implications for Pathophysiology.
G. G. Somjen (2002)
Neuroscientist 8, 254-267
   Abstract »    PDF »
Salicylate 5-Hydroxylase from Ralstonia sp. Strain U2: a Monooxygenase with Close Relationships to and Shared Electron Transport Proteins with Naphthalene Dioxygenase.
N.-Y. Zhou, J. Al-Dulayymi, M. S. Baird, and P. A. Williams (2002)
J. Bacteriol. 184, 1547-1555
   Abstract »    Full Text »    PDF »
Directed Evolution of Toluene ortho-Monooxygenase for Enhanced 1-Naphthol Synthesis and Chlorinated Ethene Degradation.
K. A. Canada, S. Iwashita, H. Shim, and T. K. Wood (2002)
J. Bacteriol. 184, 344-349
   Abstract »    Full Text »    PDF »
Laboratory Evolution of Toluene Dioxygenase To Accept 4-Picoline as a Substrate.
T. Sakamoto, J. M. Joern, A. Arisawa, and F. H. Arnold (2001)
Appl. Envir. Microbiol. 67, 3882-3887
   Abstract »    Full Text »    PDF »
Cloning and Expression of a Ralstonia eutropha HF39 Gene Mediating Indigo Formation in Escherichia coli.
S. Drewlo, C. O. Bramer, M. Madkour, F. Mayer, and A. Steinbuchel (2001)
Appl. Envir. Microbiol. 67, 1964-1969
   Abstract »    Full Text »    PDF »
Biosynthesis of Polyketides in Heterologous Hosts.
B. A. Pfeifer and C. Khosla (2001)
Microbiol. Mol. Biol. Rev. 65, 106-118
   Abstract »    Full Text »    PDF »
Substrate Specificity of Naphthalene Dioxygenase: Effect of Specific Amino Acids at the Active Site of the Enzyme.
R. E. Parales, K. Lee, S. M. Resnick, H. Jiang, D. J. Lessner, and D. T. Gibson (2000)
J. Bacteriol. 182, 1641-1649
   Abstract »    Full Text »    PDF »
Complete Sequence of a 184-Kilobase Catabolic Plasmid from Sphingomonas aromaticivorans F199.
M. F. Romine, L. C. Stillwell, K.-K. Wong, S. J. Thurston, E. C. Sisk, C. Sensen, T. Gaasterland, J. K. Fredrickson, and J. D. Saffer (1999)
J. Bacteriol. 181, 1585-1602
   Abstract »    Full Text »    PDF »
The phn Genes of Burkholderia sp. Strain RP007 Constitute a Divergent Gene Cluster for Polycyclic Aromatic Hydrocarbon Catabolism.
A. D. Laurie and G. Lloyd-Jones (1999)
J. Bacteriol. 181, 531-540
   Abstract »    Full Text »    PDF »
Plasmids Responsible for Horizontal Transfer of Naphthalene Catabolism Genes between Bacteria at a Coal Tar-Contaminated Site Are Homologous to pDTG1 from Pseudomonas putida NCIB 9816-4.
K. G. Stuart-Keil, A. M. Hohnstock, K. P. Drees, J. B. Herrick, and E. L. Madsen (1998)
Appl. Envir. Microbiol. 64, 3633-3640
   Abstract »    Full Text »    PDF »
Genetic Analysis of Dioxin Dioxygenase of Sphingomonas sp. Strain RW1: Catabolic Genes Dispersed on the Genome.
J. Armengaud, B. Happe, and K. N. Timmis (1998)
J. Bacteriol. 180, 3954-3966
   Abstract »    Full Text »    PDF »
Towards a Biocatalyst for (S)-Styrene Oxide Production: Characterization of the Styrene Degradation Pathway of Pseudomonas sp. Strain VLB120.
S. Panke, B. Witholt, A. Schmid, and M. G. Wubbolts (1998)
Appl. Envir. Microbiol. 64, 2032-2043
   Abstract »    Full Text »    PDF »
Substrate Specificities of Hybrid Naphthalene and 2,4-Dinitrotoluene Dioxygenase Enzyme Systems.
R. E. Parales, M. D. Emig, N. A. Lynch, and D. T. Gibson (1998)
J. Bacteriol. 180, 2337-2344
   Abstract »    Full Text »    PDF »
A Gene Cluster Encoding Steps in Conversion of Naphthalene to Gentisate in Pseudomonas sp. Strain U2.
S. L. Fuenmayor, M. Wild, A. L. Boyes, and P. A. Williams (1998)
J. Bacteriol. 180, 2522-2530
   Abstract »    Full Text »    PDF »
A bacterial basic region leucine zipper histidine kinase regulating toluene degradation.
P. C. K. Lau, Y. Wang, A. Patel, D. Labbe, H. Bergeron, R. Brousseau, Y. Konishi, and M. Rawlings (1997)
PNAS 94, 1453-1458
   Abstract »    Full Text »    PDF »
The Broad Substrate Chlorobenzene Dioxygenase and cis-Chlorobenzene Dihydrodiol Dehydrogenase of Pseudomonas sp. Strain P51 Are Linked Evolutionarily to the Enzymes for Benzene and Toluene Degradation.
C. Werlen, H.-P. E. Kohler, and J. R. van der Meer (1996)
J. Biol. Chem. 271, 4009-4016
   Abstract »    Full Text »    PDF »
Toward a science of metabolic engineering.
J. Bailey (1991)
Science 252, 1668-1675
   Abstract »    PDF »
Genetic engineering of bacteria from managed and natural habitats.
S. Lindow, N. Panopoulos, and B. McFarland (1989)
Science 244, 1300-1307
   Abstract »    PDF »
Production of 2-Keto-L-Gulonate, an Intermediate in L-Ascorbate Synthesis, by a Genetically Modffied Erwinia herbicola.
S. Anderson, C. B. Marks, R. Lazarus, J. Miller, K. Stafford, J. Seymour, D. Light, W. Rastetter, and D. Estell (1985)
Science 230, 144-149
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)