Science, Vol 222, Issue 4619, 72-74
Copyright © 1983 by American Association for the Advancement of Science
Segregation and mapping analysis of polymorphic HLA class I restriction fragments: detection of a novel fragment
HA Erlich,
D Stetler,
R Sheng-Dong,
D Ness,
and
C Grumet
An HLA-B7 complementary DNA clone was used as a hybridization probe to analyze the segregation pattern of polymorphic class I restriction fragments in several families whose HLA types had been determined by serological techniques. In one family in which a crossover in the HLA region had occurred, a specific genomic fragment was mapped with respect to the crossover. In another family, a novel genomic fragment present in one child and absent in all other family members was observed. With the exception of this novel fragment, all polymorphic class I fragments observed in this study segregated with a serologically defined parental haplotype, a result consistent with HLA linkage.
