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Science 2 September 1983:
Vol. 221. no. 4614, pp. 962 - 964
DOI: 10.1126/science.6879194
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Articles

Science, Vol 221, Issue 4614, 962-964
Copyright © 1983 by American Association for the Advancement of Science

articles

Isolation of lamellar bodies from neonatal mouse epidermis by selective sequential filtration

S Grayson, AD Johnson-Winegar, and PM Elias

Isolation of epidermal lamellar bodies has presented a challenge because pressures required to homogenize keratinocytes can destroy these organelles and because the lamellar body readily releases its contents during prolonged isolation procedures. In an attempt to isolate lamellar bodies, sheets of intact stratum corneum and stratum granulosum were obtained from neonatal mice with highly purified staphylococcal epidermolytic toxin, disrupted, and passed through a series of filters. The final filtrate was rich in intact lamellar bodies and contained variable amounts of ribosomes and other vesicular structures. Availability of a highly purified lamellar body preparation from postnatal epidermis should help to clarify the role of this organelle in epidermal function. The technique of selective, sequential filtration represents a new approach to cell fractionation that may have wide applications in cell biology and biochemistry.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
ß-Glucocerebrosidase activity in mammalian stratum corneum.
Y. Takagi, E. Kriehuber, G. Imokawa, P. M. Elias, and W. M. Holleran (1999)
J. Lipid Res. 40, 861-869
   Abstract »    Full Text »
Ichthyosis Induced by Cholesterol-Lowering Drugs: Implications for Epidermal Cholesterol Homeostasis.
M. L. Williams, K. R. Feingold, G. Grubauer, and P. M. Elias (1987)
Arch Dermatol 123, 1535-1538
   Abstract »    PDF »


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