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Science 19 August 1983:
Vol. 221. no. 4612, pp. 769 - 770
DOI: 10.1126/science.6308761
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Articles

Science, Vol 221, Issue 4612, 769-770
Copyright © 1983 by American Association for the Advancement of Science

articles

Nitrite inhibition of Clostridium botulinum: electron spin resonance detection of iron-nitric oxide complexes

D Reddy, JR Lancaster Jr, and DP Cornforth

Vegetative cells of Clostridium botulinum were shown to contain iron-sulfur proteins that react with added nitrite to form iron-nitric oxide complexes, with resultant destruction of the iron-sulfur cluster. Inactivation of iron-sulfur enzymes (especially ferredoxin) by binding of nitric oxide would almost certainly inhibit growth, and thus is probably the mechanism of botulinal inhibition by nitrite in foods.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Femtomolar Sensitivity of a NO Sensor from Clostridium botulinum.
P. Nioche, V. Berka, J. Vipond, N. Minton, A.-L. Tsai, and C. S. Raman (2004)
Science 306, 1550-1553
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Non-heme iron protein: A potential target of nitric oxide in acute cardiac allograft rejection.
G. M. Pieper, N. L. N. Halligan, G. Hilton, E. A. Konorev, C. C. Felix, A. M. Roza, M. B. Adams, and O. W. Griffith (2003)
PNAS 100, 3125-3130
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Direct nitric oxide signal transduction via nitrosylation of iron-sulfur centers in the SoxR transcription activator.
H. Ding and B. Demple (2000)
PNAS 97, 5146-5150
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Nitric Oxide Modulates Mitochondrial Respiration in Failing Human Heart.
K. E. Loke, S. K. Laycock, S. Mital, M. S. Wolin, R. Bernstein, M. Oz, L. Addonizio, G. Kaley, and T. H. Hintze (1999)
Circulation 100, 1291-1297
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Characterization of the Bactericidal Effects of Sodium Nitroprusside and Other Pentacyanonitrosyl Complexes on the Food Spoilage Bacterium Clostridium sporogenes.
C. L. Joannou, X.-Y. Cui, N. Rogers, N. Vielotte, C. L. Torres Martinez, N. V. Vugman, M. N. Hughes, and R. Cammack (1998)
Appl. Envir. Microbiol. 64, 3195-3201
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Helicobacter pylori Generates Superoxide Radicals and Modulates Nitric Oxide Metabolism.
K. Nagata, H. Yu, M. Nishikawa, M. Kashiba, A. Nakamura, E. F. Sato, T. Tamura, and M. Inoue (1998)
J. Biol. Chem. 273, 14071-14073
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An EPR Investigation of the Products of the Reaction of Cytosolic and Mitochondrial Aconitases with Nitric Oxide.
M. C. Kennedy, W. E. Antholine, and H. Beinert (1997)
J. Biol. Chem. 272, 20340-20347
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Nitric Oxide Prevents Oxidative Damage Produced by tert-Butyl Hydroperoxide in Erythroleukemia Cells via Nitrosylation of Heme and Non-heme Iron. ELECTRON PARAMAGNETIC RESONANCE EVIDENCE.
N. V. Gorbunov, J. C. Yalowich, A. Gaddam, P. Thampatty, V. B. Ritov, E. R. Kisin, N. M. Elsayed, and V. E. Kagan (1997)
J. Biol. Chem. 272, 12328-12341
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Reaction of Human Myoglobin and Nitric Oxide. HEME IRON OR PROTEIN SULFHYDRYL (S) NITROSATION DEPENDENCE ON THE ABSENCE OR PRESENCE OF OXYGEN.
P. K. Witting, D. J. Douglas, and A. G. Mauk (2001)
J. Biol. Chem. 276, 3991-3998
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L-Cysteine-mediated Destabilization of Dinitrosyl Iron Complexes in Proteins.
P. A. Rogers and H. Ding (2001)
J. Biol. Chem. 276, 30980-30986
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