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Science 5 August 1983:
Vol. 221. no. 4610, pp. 551 - 553
DOI: 10.1126/science.6306768

Articles

Science, Vol 221, Issue 4610, 551-553
Copyright © 1983 by American Association for the Advancement of Science


articles

High efficiency DNA-mediated transformation of primate cells

C Gorman, R Padmanabhan, and BH Howard

Tissue culture cells from several mammalian species, including three primate lines, were transfected with recombinant vectors carrying Escherichia coli xanthine-guanine phosphoribosyltransferase or Tn5 aminoglycoside phosphotransferase dominant selectable markers. Human HeLa and SV40-transformed xeroderma pigmentosum cells exhibited stable transformation frequencies of at least 10(-3) (0.1 percent). CV-1, an African green monkey kidney cell line, could be stably transformed with the exceptionally high frequency of 6 X 10(-2) (6 percent).


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A 300 bp 5'-upstream sequence of a differentiation-dependent rabbit K3 keratin gene can serve as a keratinocyte-specific promoter.
R. Wu, S Galvin, S. Wu, C Xu, M Blumenberg, and T. Sun (1993)
J. Cell Sci. 105, 303-316
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The block to transcriptional elongation within the human c-myc gene is determined in the promoter-proximal region..
A Krumm, T Meulia, M Brunvand, and M Groudine (1992)
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A Leask, M Rosenberg, R Vassar, and E Fuchs (1990)
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C A Spencer, R C LeStrange, U Novak, W S Hayward, and M Groudine (1990)
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Histone H5 in the control of DNA synthesis and cell proliferation.
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Identification and Characterization of Cell Lines with a Defect in a Post-adsorption Stage of Sendai Virus-mediated Membrane Fusion.
A. Eguchi, T. Kondoh, H. Kosaka, T. Suzuki, H. Momota, A. Masago, T. Yoshida, H. Taira, A. Ishii-Watabe, J. Okabe, et al. (2000)
J. Biol. Chem. 275, 17549-17555
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