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Science 22 July 1983:
Vol. 221. no. 4608, pp. 366 - 368
DOI: 10.1126/science.221.4608.366
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Articles

Intracellular pH Regulates Transitions Between Dormancy and Development of Brine Shrimp (Artemia salina) Embryos

WILLIAM B. BUSA 1 and JOHN H. CROWE 1

1 Department of Zoology, University of California, Davis 95616

The intracellular pH (pHi)of encysted gastrula-stage embryos of the brine shrip, Artemia, as previously shown by in vivo phosphorus-31 nuclear magnetic resonance spectroscopy, increases by more than 1 unit during arousal from cryptobiotic dormancy and decreases by the same amount during reinduction of dormancy. These changes in pHi are now shown to be a fundamental regulator of the transitions between dormancy and metabolism: acidification of activated embryo pHi by more than 1 unit with carbon dioxide induced a state comparable to natural dormancy, while alkalinization of dormant embryo pHi with the weak base ammonia terminated natural dormancy. This demonstration of pHi-mediated regulation of cryptobiotic dormancy extends the known scope for pHi as a regulator of development to include multicellular stages of the metazoan life cycle.

Submitted on January 25, 1983
Revised on March 15, 1983


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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