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Science 24 June 1983:
Vol. 220. no. 4604, pp. 1394 - 1396
DOI: 10.1126/science.6857258
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Articles

Science, Vol 220, Issue 4604, 1394-1396
Copyright © 1983 by American Association for the Advancement of Science

articles

Serum factor supporting long-term survival of rat central neurons in culture

LM Kaufman and JN Barrett

Gel filtration of serum at pH 3.6 yielded a fraction that supported long-term (months) survival of dissociated rat central neurons in monolayer culture more reliably than the traditionally used unfractionated serum. The cultures remained neuron-rich, because this fraction did not support the proliferation of glia and fibroblasts that occurs in whole serum. With an apparent molecular weight of 55,000 and an isoelectric point of 5.6, the active factor (or factors) in this fraction is distinct from any well-defined growth factor.


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