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Science 13 May 1983:
Vol. 220. no. 4598, pp. 725 - 727
DOI: 10.1126/science.6301016
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Articles

Science, Vol 220, Issue 4598, 725-727
Copyright © 1983 by American Association for the Advancement of Science

articles

Gene transfer by polyoma-like particles assembled in a cell-free system

SN Slilaty and HV Aposhian

Empty capsids of polyoma virus interact with DNA in a cell-free system to form polyoma-like particles (PLP). The DNA in these particles is protected from the action of pancreatic deoxyribonuclease. Transfer of genetic information by PLP has been accomplished by using a restriction fragment containing the transforming sequences of polyoma DNA as a model gene. Infection of rat F111 cells by PLP containing these sequences results in DNA-mediated cellular transformation. Gene transfer by PLP is 50 to 150 times more efficient than by the calcium phosphate precipitation method.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)