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Science 13 May 1983:
Vol. 220. no. 4598, pp. 723 - 725
DOI: 10.1126/science.6836308
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Articles

Science, Vol 220, Issue 4598, 723-725
Copyright © 1983 by American Association for the Advancement of Science

articles

Formation of an adenine-thymine photoadduct in the deoxydinucleoside monophosphate d(TpA) and in DNA

SN Bose, RJ Davies, SK Sethi, and JA McCloskey

A photoadduct is formed between the adenine (A) and thymine (T) bases of the deoxydinucleoside monophosphate d(TpA) when it is irradiated at 254 nanometers in aqueous solution. Treatment of the photoadduct with acid converts it specifically into a fluorescent hydrolysis product, C7H7N3O, incorporating the position-8 carbon of adenine and the methyl group of thymine. Isolation of the fluorescent hydrolysis product from acid hydrolyzates of oligo- and polydeoxyribonucleotides has shown that the photoadduct is formed by ultraviolet irradiation of d(pTpA), d(TpApT), d(TpApTpA), poly(dA-dT), and both single- and double-stranded DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Photocrosslinking of human telomeric G-quadruplex loops by anti cyclobutane thymine dimer formation.
D. G. T. Su, H. Fang, M. L. Gross, and J.-S. A. Taylor (2009)
PNAS 106, 12861-12866
   Abstract »    Full Text »    PDF »
High-resolution crystal structure of the intramolecular d(TpA) thymine-adenine photoadduct and its mechanistic implications.
R. J. H. Davies, J. F. Malone, Y. Gan, C. J. Cardin, M. P.H. Lee, and S. Neidle (2007)
Nucleic Acids Res. 35, 1048-1053
   Abstract »    Full Text »    PDF »
Efficiency and pattern of UV pulse laser-induced RNA-RNA cross-linking in the ribosome.
T. Shapkina, S. Lappi, S. Franzen, and P. Wollenzien (2004)
Nucleic Acids Res. 32, 1518-1526
   Abstract »    Full Text »    PDF »


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