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Science 6 May 1983:
Vol. 220. no. 4597, pp. 620 - 622
DOI: 10.1126/science.6188215
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Articles

Science, Vol 220, Issue 4597, 620-622
Copyright © 1983 by American Association for the Advancement of Science

articles

Bacterial characterization by flow cytometry

MA Van Dilla, RG Langlois, D Pinkel, D Yajko, and WK Hadley

Bacteria were analyzed in a dual-beam flow cytometer after double staining with the fluorescent dyes chromomycin A3 and Hoechst 33258, which bind preferentially to DNA that is rich in guanine-cytosine and adenine-thymine, respectively. The measurements were indicative of the cellular DNA content and base composition, cell concentration, and proliferative state of the population. The ratio of the chromomycin A3 signal to the Hoechst 33258 signal increased with the guanine-cytosine content of the cellular DNA for the six cultured species measured, following expectation. Bacteria in urine from patients with urinary tract infections were characterized without interference from host cell DNA, debris, or other particulates.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Determination of Total Protein Content of Bacterial Cells by SYPRO Staining and Flow Cytometry.
M. V. Zubkov, B. M. Fuchs, H. Eilers, P. H. Burkill, and R. Amann (1999)
Appl. Envir. Microbiol. 65, 3251-3257
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Gigantism in a Bacterium, Epulopiscium fishelsoni, Correlates with Complex Patterns in Arrangement, Quantity, and Segregation of DNA.
V. Bresler, W. L. Montgomery, L. Fishelson, and P. E. Pollak (1998)
J. Bacteriol. 180, 5601-5611
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Determination of the Biomasses of Small Bacteria at Low Concentrations in a Mixture of Species with Forward Light Scatter Measurements by Flow Cytometry.
B. R. Robertson, D. K. Button, and A. L. Koch (1998)
Appl. Envir. Microbiol. 64, 3900-3909
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Characteristics and Dynamics of Bacterial Populations during Postantibiotic Effect Determined by Flow Cytometry.
M. Gottfredsson, H. Erlendsdóttir, A. Sigfússon, and S. Gudmundsson (1998)
Antimicrob. Agents Chemother. 42, 1005-1011
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Automated Immunofluorescent Speciation of Oral Bacteria Using Flow Cytometry.
J.M. Barnett, M.A. Cuchens, and W. Buchanan (1984)
Journal of Dental Research 63, 1040-1042
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