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Science 6 May 1983:
Vol. 220. no. 4597, pp. 615 - 617
DOI: 10.1126/science.6836305
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Articles

Science, Vol 220, Issue 4597, 615-617
Copyright © 1983 by American Association for the Advancement of Science

articles

Metastable species of hemoglobin: room temperature transients and cryogenically trapped intermediates

MR Ondrias, JM Friedman, and DL Rousseau

Resonance Raman spectra of photolyzed carbonmonoxyhemoglobin obtained with 10-nanosecond pulses are compared with the spectra of photolyzed carbonmonoxyhemoglobin stabilized at 80 K. In comparing the deoxy with the photodissociated species, the changes in the Raman spectra are the same for these two experimental regimes. These results show that at ambient and cryogenic temperatures the heme pocket in liganded hemoglobin is significantly different from that of deoxyhemoglobin. It is concluded that measurements of the properties of intermediate species from photodissociated hemoglobin stabilized at low temperatures can be used to probe the short-lived metastable forms of hemoglobin present after photodissociation under biologically relevant solution conditions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Structure, dynamics, and reactivity in hemoglobin.
J. Friedman (1985)
Science 228, 1273-1280
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