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Science 29 April 1983:
Vol. 220. no. 4596, pp. 515 - 517
DOI: 10.1126/science.6403984
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Articles

Science, Vol 220, Issue 4596, 515-517
Copyright © 1983 by American Association for the Advancement of Science

articles

Cloned fragment A of diphtheria toxin is expressed and secreted into the periplasmic space of Escherichia coli K12

D Leong, KD Coleman, and Murphy JR

An 831-base pair segment of the corynebacteriophage beta tox-45 genome encoding fragment A of diphtheria toxin was cloned into plasmid pUC8 in Escherichia coli K12. Strains containing recombinant plasmids expressed the adenosine diphosphate ribosyl transferase activity characteristic of fragment A; this activity could be inhibited by polyvalent antiserum to fragment A as well as by the appropriate monoclonal antibodies to diphtheria toxin. The transferase activity was secreted into the periplasmic space of E. coli. These findings have implications for the future construction of genetically engineered chimeric toxins.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Transgenic Mice Expressing a Fully Nontoxic Diphtheria Toxin Mutant, not CRM197 Mutant, Acquire Immune Tolerance against Diphtheria Toxin.
Y. Kimura, M. Saito, Y. Kimata, and K. Kohno (2007)
J. Biochem. 142, 105-112
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Prostate-specific Expression of the Diphtheria Toxin A Chain (DT-A): Studies of Inducibility and Specificity of Expression of Prostate-specific Antigen Promoter-driven DT-A Adenoviral-mediated Gene Transfer.
Y. Li, J. McCadden, F. Ferrer, M. Kruszewski, M. Carducci, J. Simons, and R. Rodriguez (2002)
Cancer Res. 62, 2576-2582
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Nucleotide sequence and expression of the diphtheria tox228 gene in Escherichia coli.
M Kaczorek, F Delpeyroux, N Chenciner, R. Streeck, Murphy JR, P Boquet, and P Tiollais (1983)
Science 221, 855-858
   Abstract »    PDF »
Delivery of a stringent dimerizer-regulated gene expression system in a single retroviral vector.
R. Pollock, R. Issner, K. Zoller, S. Natesan, V. M. Rivera, and T. Clackson (2000)
PNAS 97, 13221-13226
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