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Science 29 April 1983:
Vol. 220. no. 4596, pp. 498 - 500
DOI: 10.1126/science.6836293
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Articles

Science, Vol 220, Issue 4596, 498-500
Copyright © 1983 by American Association for the Advancement of Science

articles

Electron microscope tomography: transcription in three dimensions

DE Olins, AL Olins, HA Levy, RC Durfee, SM Margle, EP Tinnel, and SD Dover

Three-dimensional reconstruction of an asymmetric biological ultrastructure has been achieved by tomographic analysis of electron micrographs of sections tilted on a goniometer specimen stage. Aligned micrographs could be displayed as red-green three-dimensional movies. The techniques have been applied to portions of in situ transcription units of a Balbiani ring in the polytene chromosomes of the midge Chironomus tentans. Current data suggest a DNA compaction of about 8 to 1 in a transcription unit. Nascent ribonucleoprotein granules display an imperfect sixfold helical arrangement around the chromatin axis.


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Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter.
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J. Cell Biol. 154, 33-48
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Electron Microscopy of Cells: A New Beginning for a New Century.
J. R. McIntosh (2001)
J. Cell Biol. 153, f25-f32
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Parallel Computation of a Maximum-Likelihood Estimator of a Physical Map.
S. M. Bhandarkar, S. A. Machaka, S. S. Shete, and R. N. Kota (2001)
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GABAA Receptor Assembly. IDENTIFICATION AND STRUCTURE OF gamma 2 SEQUENCES FORMING THE INTERSUBUNIT CONTACTS WITH alpha 1 AND beta 3 SUBUNITS.
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