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Science 11 February 1983:
Vol. 219. no. 4585, pp. 683 - 688
DOI: 10.1126/science.219.4585.683
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Articles

Genetic Transfer in Plants Through Interspecific Protoplast Fusion

James F. Shepard 1, Dennis Bidney 2, Tina Barsby 3, and Roger Kemble 4

1 Professor in the Department of Plant Pathology, Kansas State University, Manhattan 66506
2 Research scientist with Advanced Genetic Sciences, Inc., P.O. Box 1373, Manhattan, Kansas 66502
3 Postdoctoral associate in the Department of Plant Pathology, Kansas State University, Manhattan 66506
4 Assistant professor in the Department of Plant Pathology, Kansas State University, Manhattan 66506

Protoplasts of sexually incompatible species have been fused and in some combinations have given rise to somatic hybrid plants. Partial elimination of parental chromosomes from either species is common in such hybrids, but total chromosome loss has generally occurred only with phylogenetically unrelated pairings. Genetic function of one parent may be retained despite a complete loss of its chromosomes, suggesting that genetic introgression is possible in the absence of complete donor chromosomes. A model interspecific combination for such studies is the potato-tomato somatic hybrid for which numerous phenotypes and karyotypes are encountered at the outset, with a broader range observed in the second somatic generation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Isolation of Agronomically Useful Mutants from Plant Cell Cultures.
R. S. Chaleff (1983)
Science 219, 676-682
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