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Science 14 January 1983:
Vol. 219. no. 4581, pp. 176 - 178
DOI: 10.1126/science.6849127
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Articles

Science, Vol 219, Issue 4581, 176-178
Copyright © 1983 by American Association for the Advancement of Science

articles

Quaternary and quinternary structures of native chromatin DNA in liver nuclei: differential scanning calorimetry

C Nicolini, V Trefiletti, B Cavazza, C Cuniberti, E Patrone, P Carlo, and G Brambilla

Differential scanning calorimetry of chromatin isolated from rat liver cells revealed three discrete thermal transitions whose temperatures and melting enthalpies depend on ionic strength in the range 0 to 600 millimolar NaCl. Intact nuclei showed a fourth thermal transition at a lower temperature and different melting enthalpies for the other three transitions still present at temperatures similar to those obtained in isolated chromatin. The data are discussed in terms of the tertiary, quaternary, and quinternary structures of chromatin DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Condensation of Chromatin in Apoptotic Thymocytes Shows a Specific Structural Change.
C. Allera, G. Lazzarini, E. Patrone, I. Alberti, P. Barboro, P. Sanna, A. Melchiori, S. Parodi, and C. Balbi (1997)
J. Biol. Chem. 272, 10817-10822
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