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Science 10 December 1982:
Vol. 218. no. 4577, pp. 1122 - 1125
DOI: 10.1126/science.6293052
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Articles

Science, Vol 218, Issue 4577, 1122-1125
Copyright © 1982 by American Association for the Advancement of Science

articles

New method for detecting cellular transforming genes

DG Blair, CS Cooper, MK Oskarsson, LA Eader, and GF Vande Woude

Tumor induction in athymic nude mice can be used to detect dominant transforming genes in cellular DNA. Mouse NIH 3T3 cells freshly transfected with either cloned Moloney sarcoma proviral DNA or cellular DNA's derived from virally transformed cells induced tumors when injected into athymic nu/nu mice. Tumors were also induced by cells transfected with DNA from two tumor-derived and one chemically transformed human cell lines. The mouse tumors induced by human cell line DNA's contained human DNA sequences, and DNA derived from these tumors was capable of inducing both tumors and foci on subsequent transfection. Tumor induction in nude mice represents a useful new method for the detection and selection of cells transformed by cellular oncogenes.


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