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Science 15 October 1982:
Vol. 218. no. 4569, pp. 290 - 291
DOI: 10.1126/science.7123234
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Articles

Science, Vol 218, Issue 4569, 290-291
Copyright © 1982 by American Association for the Advancement of Science

articles

Biospecific labeling with undecagold: visualization of the biotin-binding site on avidin

D Safer, J Hainfeld, JS Wall, and JE Reardon

The biotin-binding site on avidin has been labeled with biotin conjugated to undecagold, an organometallic cluster compound containing 11 gold atoms in a core angestroms in diameter. Examination of unstained specimens by scanning transmission electron microscopy reveals the labeled sites directly, without computational averaging or filtering of the images. This approach should be widely applicable for determining the locations of subunits and functional site in biological macromolecules at a resolution at a resolution in range of 15 angstroms.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A small gold-conjugated antibody label: improved resolution for electron microscopy.
J. Hainfeld (1987)
Science 236, 450-453
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