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Science 8 October 1982:
Vol. 218. no. 4568, pp. 162 - 165
DOI: 10.1126/science.6981844
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Articles

Science, Vol 218, Issue 4568, 162-165
Copyright © 1982 by American Association for the Advancement of Science

articles

Complete amino acid sequence of urotensin I, a hypotensive and corticotropin-releasing neuropeptide from Catostomus

K Lederis, A Letter, D McMaster, G Moore, and D Schlesinger

Urotensin I, purified from extracts of the urophysis of a teleost fish (Catostomus commersoni), exhibits potent hypotensive activity (mammals and birds) and corticotropin-releasing activity (both fish and mammals). The primary structure of this 41-residue peptide was determined to be H-Asn-Asp-Asp-Pro-Pro-Ile-Ser-Ile-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Asn-Met- Ile-Glu- Met-Ala-Arg-Ile-Glu-Asn-Glu-Arg-Glu-Gln-Ala-Gly-Leu-Asn-Arg-Lys-Tyr-Leu-As p-Glu -Val-NH2. Extraction with 0.1N HCl at 100 degrees C cleaves the amino-terminal tripeptide, yeilding a fully active analog, urotensin I(4-41). The amino acid sequence was confirmed by measuring the biological activity of synthetic urotensin I(4-41). Urotensin I exhibits a striking sequence homology with ovine corticotropin-releasing factor and with frog sauvagine. These three peptides exhibit similar activities in biological test systems.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Residue 17 of Sauvagine Cross-links to the First Transmembrane Domain of Corticotropin-releasing Factor Receptor 1 (CRFR1).
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J. Biol. Chem. 283, 35644-35651
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Urocortins of the South African Clawed Frog, Xenopus laevis: Conservation of Structure and Function in Tetrapod Evolution.
G. C. Boorse, E. J. Crespi, F. M. Dautzenberg, and R. J. Denver (2005)
Endocrinology 146, 4851-4860
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Coexpression of Corticotropin-Releasing Hormone and Urotensin I Precursor Genes in the Caudal Neurosecretory System of the Euryhaline Flounder (Platichthys flesus): A Possible Shared Role in Peripheral Regulation.
W. Lu, L. Dow, S. Gumusgoz, M. J. Brierley, J. M. Warne, C. R. McCrohan, R. J. Balment, and D. Riccardi (2004)
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Promoter Analysis of Human Corticotropin-Releasing Factor (CRF) Type 1 Receptor and Regulation by CRF and Urocortin.
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Relaxation by urocortin of rat renal arteries: effects of diabetes in males and females.
E. Sanz, N. Fernandez, L. Monge, B. Climent, G. Dieguez, and A. L. Garcia-Villalon (2003)
Cardiovasc Res 58, 706-711
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International Union of Pharmacology. XXXVI. Current Status of the Nomenclature for Receptors for Corticotropin-Releasing Factor and Their Ligands.
R. L. Hauger, D. E. Grigoriadis, M. F. Dallman, P. M. Plotsky, W. W. Vale, and F. M. Dautzenberg (2003)
Pharmacol. Rev. 55, 21-26
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Five Amino Acids of the Xenopus laevis CRF (Corticotropin-Releasing Factor) Type 2 Receptor Mediate Differential Binding of CRF Ligands in Comparison with Its Human Counterpart.
F. M. Dautzenberg, J. Higelin, O. Brauns, B. Butscha, and R. L. Hauger (2002)
Mol. Pharmacol. 61, 1132-1139
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N-glycosylation of CRF receptor type 1 is important for its ligand-specific interaction.
I. Q. Assil and A. B. Abou-Samra (2001)
Am J Physiol Endocrinol Metab 281, E1015-E1021
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Bursting properties of caudal neurosecretory cells in the flounder Platichthys flesus, in vitro.
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Characterization of Three Corticotropin-Releasing Factor Receptors in Catfish: A Novel Third Receptor Is Predominantly Expressed in Pituitary and Urophysis.
M. Arai, I. Q. Assil, and A. B. Abou-Samra (2001)
Endocrinology 142, 446-454
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Peripheral urocortin delays gastric emptying: role of CRF receptor 2.
T. Nozu, V. Martinez, J. Rivier, and Y. Tache (1999)
Am J Physiol Gastrointest Liver Physiol 276, G867-G874
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Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord.
Y. Coulouarn, I. Lihrmann, S. Jegou, Y. Anouar, H. Tostivint, J. C. Beauvillain, J. M. Conlon, H. A. Bern, and H. Vaudry (1998)
PNAS 95, 15803-15808
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Vasodilator Actions of Urocortin and Related Peptides in the Human Perfused Placenta in Vitro.
I. M. Leitch, A. L. A. Boura, C. Botti, M. A. Read, W. A. W. Walters, and R. Smith (1998)
J. Clin. Endocrinol. Metab. 83, 4510-4513
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Mapping of the ligand-selective domain of the Xenopus laevis corticotropin-releasing factor receptor 1: Implications for the ligand-binding site.
F. M. Dautzenberg, S. Wille, R. Lohmann, and J. Spiess (1998)
PNAS 95, 4941-4946
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Localization of Agonist- and Antagonist-Binding Domains of Human Corticotropin-Releasing Factor Receptors.
C. W. Liaw, D. E. Grigoriadis, M. T. Lorang, E. B. De Souza, and R. A. Maki (1997)
Mol. Endocrinol. 11, 2048-2053
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Clinical Applications of Corticotropin-Releasing Factor.
G. P. CHROUSOS, T. H. SCHUERMEYER, J. DOPPMAN, E. H. OLDFIELD, H. M. SCHULTE, P. W. GOLD, and D. L. LORIAUX (1985)
Ann Intern Med 102, 344-358
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Synthetic competitive antagonists of corticotropin-releasing factor: effect on ACTH secretion in the rat.
J Rivier, C Rivier, and W Vale (1984)
Science 224, 889-891
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