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Science 17 September 1982:
Vol. 217. no. 4565, pp. 1145 - 1146
DOI: 10.1126/science.6180477
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Articles

Science, Vol 217, Issue 4565, 1145-1146
Copyright © 1982 by American Association for the Advancement of Science

articles

High efficiency latency and activation of herpes simplex virus in human cells

BL Wigdahl, AC Scheck, E De Clercq, and F Rapp

Herpes simplex virus (HSV) exists in humans in a latent form that can be activated. To characterize the molecular basis of the cell-virus interactions and to analyze the state of the latent HSV genome, an in vitro model system was established. In this system a large fraction of the latently infected cells contain an HSV genome that can be activated. Cell survival was reduced minimally after repression of high multiplicity HSV type 1 (HSV-1) infection of human fibroblast cells with (E)-5-(2-bromovinyl)-2'-deoxyuridine in combination with human leukocyte interferon (IFN-alpha). A minimum of 1 to 3 percent of the surviving cells contained an HSV genome that could be activated either by human cytomegalovirus superinfection or reduction in incubation temperature.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Efficient Quiescent Infection of Normal Human Diploid Fibroblasts with Wild-Type Herpes Simplex Virus Type 1.
R. McMahon and D. Walsh (2008)
J. Virol. 82, 10218-10230
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Reactivation of Expression from Quiescent Herpes Simplex Virus Type 1 Genomes in the Absence of Immediate-Early Protein ICP0.
C. M. Preston (2007)
J. Virol. 81, 11781-11789
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Human Cytomegalovirus Tegument Protein pp71 Directs Long-Term Gene Expression from Quiescent Herpes Simplex Virus Genomes.
C. M. Preston and M. J. Nicholl (2005)
J. Virol. 79, 525-535
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Persistence of Infectious Herpes Simplex Virus Type 2 in the Nervous System in Mice after Antiviral Chemotherapy.
A. M. Thackray and H. J. Field (2000)
Antimicrob. Agents Chemother. 44, 97-102
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Molecular Aspects of Herpes Simplex Virus I Latency, Reactivation, and Recurrence.
C.S. Miller, R.J. Danaher, and R.J. Jacob (1998)
Critical Reviews in Oral Biology & Medicine 9, 541-562
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Latent Herpesviruses of Humans.
M. C. JORDAN, G. W. JORDAN, J. G. STEVENS, and G. MILLER (1984)
Ann Intern Med 100, 866-880
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