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Science 3 September 1982:
Vol. 217. no. 4563, pp. 950 - 952
DOI: 10.1126/science.7112108
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Articles

Science, Vol 217, Issue 4563, 950-952
Copyright © 1982 by American Association for the Advancement of Science

articles

Initiation of endochondral calcification is related to changes in the redox state of hypertrophic chondrocytes

IM Shapiro, EE Golub, S Kakuta, J Hazelgrove, J Havery, B Chance, and P Frasca

The level of pyridine nucleotides (NADH and NAD+) in the mineralizing growth plate of the chick was ascertained by high-resolution scanning microfluorimetry and biochemical analysis. Scanning electron microscopy and light microscopy were used to relate the concentrations of NADH and NAD+ to stages of chondrocyte maturation. A dramatic increase was found in the relative concentration of reduced pyridine nucleotides in the hypertrophic zone. On either side of this zone, in proliferating and calcifying cartilage, there was a decrease in NADH fluorescence, and the NADH/NAD+ ratio was depressed. The finding that NADH accumulated in the tissue zone associated with the earliest deposition of bone mineral supports the hypothesis that a change in the redox state initiates tissue mineralization.


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Dietary Xylitol Protects Against Weakening of Bone Biomechanical Properties in Ovariectomized Rats.
P. T. Mattila, M. J. Svanberg, P. Pökkä, and M. L. E. Knuuttila (1998)
J. Nutr. 128, 1811-1814
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