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Science 3 September 1982:
Vol. 217. no. 4563, pp. 948 - 950
DOI: 10.1126/science.6287574
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Articles

Science, Vol 217, Issue 4563, 948-950
Copyright © 1982 by American Association for the Advancement of Science

articles

Phagocyte impotence caused by an invasive bacterial adenylate cyclase

DL Confer and JW Eaton

For unknown reasons, humans infected with the bacterium Bordetella pertussis are exceptionally vulnerable to secondary infections. Bordetella species elaborate a soluble, heat-stable, and highly active adenylate cyclase. This enzyme is internalized by phagocytic cells and catalyzes the unregulated formation of adenosine 3',5'-monophosphate (cyclic AMP), thereby disrupting normal cellular function. This unusual phenomenon may explain Bordetella-induced aphylaxis and may prove to be useful for investigating a variety of cyclic AMP-governed processes.


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M. Gray, G. Szabo, A. S. Otero, L. Gray, and E. Hewlett (1998)
J. Biol. Chem. 273, 18260-18267
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Role of Adenylate Cyclase-Hemolysin in Alveolar Macrophage Apoptosis during Bordetella pertussis Infection In Vivo.
P. Gueirard, A. Druilhe, M. Pretolani, and N. Guiso (1998)
Infect. Immun. 66, 1718-1725
   Abstract »    Full Text »    PDF »
Replacement of Conserved Cysteines in Human Tissue Inhibitor of Metalloproteinases-1.
N. C. M. Caterina, L. J. Windsor, A. E. Yermovsky, M. K. Bodden, K. B. Taylor, H. Birkedal-Hansen, and J. A. Engler (1997)
J. Biol. Chem. 272, 32141-32149
   Abstract »    Full Text »    PDF »
Interaction of Calcium with Bordetella pertussis Adenylate Cyclase Toxin.
T. Rose, P. Sebo, J. Bellalou, and D. Ladant (1995)
J. Biol. Chem. 270, 26370-26376
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Hemolytic, but Not Cell-invasive Activity, of Adenylate Cyclase Toxin Is Selectively Affected by Differential Fatty-acylation in Escherichia coli.
M. Hackett, C. B. Walker, L. Guo, M. C. Gray, S. Van Cuyk, A. Ullmann, J. Shabanowitz, D. F. Hunt, E. L. Hewlett, and P. Sebo (1995)
J. Biol. Chem. 270, 20250-20253
   Abstract »    Full Text »    PDF »
Membrane Depolarization Prevents Cell Invasion by Bordetella pertussis Adenylate Cyclase Toxin.
A. S. Otero, X. B. Yi, M. C. Gray, G. Szabo, and E. L. Hewlett (1995)
J. Biol. Chem. 270, 9695-9697
   Abstract »    Full Text »    PDF »
Internal lysine palmitoylation in adenylate cyclase toxin from Bordetella pertussis.
M Hackett, L Guo, J Shabanowitz, D. Hunt, and E. Hewlett (1994)
Science 266, 433-435
   Abstract »    PDF »
Cyclic Nucleotides: Mediators of Bacterial Toxin Action in Disease.
J. MOSS, D. L. BURNS, J. A. HSIA, E. L. HEWLETT, R. L. GUERRANT, and M. VAUGHAN (1984)
Ann Intern Med 101, 653-666
   Abstract »    PDF »
Acylation of Lysine 983 Is Sufficient for Toxin Activity of Bordetella pertussis Adenylate Cyclase. SUBSTITUTIONS OF ALANINE 140 MODULATE ACYLATION SITE SELECTIVITY OF THE TOXIN ACYLTRANSFERASE CyaC.
T. Basar, V. Havlicek, S. Bezouskova, M. Hackett, and P. Sebo (2001)
J. Biol. Chem. 276, 348-354
   Abstract »    Full Text »    PDF »


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