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Science 20 August 1982:
Vol. 217. no. 4561, pp. 737 - 739
DOI: 10.1126/science.6980467

Articles

Science, Vol 217, Issue 4561, 737-739
Copyright © 1982 by American Association for the Advancement of Science


articles

Transformation of human leukocytes by cocultivation with an adult T cell leukemia virus producer cell line

N Yamamoto, M Okada, Y Koyanagi, M Kannagi, and Y Hinuma

The transmission of adult T cell leukemia virus, a human retrovirus, into fresh leukocytes from normal humans was examined. One of three virus-carrying cell lines, tested after being subjected to lethal x-irradiation, consistently transformed leukocytes from adult peripheral blood and umbilical cord blood. All the transformed cell lines expressed adult T cell leukemia virus-associated antigen, but transformed lines originating from adult and umbilical cord blood exhibited T cell and non-T, non-B cell surface natures, respectively. Efforts to transform human leukocytes with cell-free virus were unsuccessful.


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