Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 23 July 1982:
Vol. 217. no. 4557, pp. 371 - 373
DOI: 10.1126/science.7046050
Prev | Table of Contents | Next

Articles

Science, Vol 217, Issue 4557, 371-373
Copyright © 1982 by American Association for the Advancement of Science

articles

Lethal disruption of the yeast actin gene by integrative DNA transformation

D Shortle, JE Haber, and D Botstein

A mutant allele of the chromosomal locus corresponding to the cloned actin gene of the yeast Saccharomyces cerevisiae has been constructed by DNA transformation with a hybrid plasmid which integrates into, and thereby disrupts, the protein-encoding sequences of the gene. In a diploid strain of yeast, disruption of the actin gene on one chromosome results in a mutation that segregates as a recessive lethal tightly linked to a selectable genetic marker on the integrated plasmid. The actin gene, therefore, must encode an essential function for yeast cell growth.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The 2009 Novitski Prize.
J. E. Haber (2009)
Genetics 181, 837-838
   Full Text »    PDF »
Functional Characterization of Pneumocystis carinii brl1 by Transspecies Complementation Analysis.
L. Lo Presti, M. Cockell, L. Cerutti, V. Simanis, and P. M. Hauser (2007)
Eukaryot. Cell 6, 2448-2452
   Abstract »    Full Text »    PDF »
Class-Specific Interaction of Profilin and ADF Isovariants with Actin in the Regulation of Plant Development.
M. K. Kandasamy, B. Burgos-Rivera, E. C. McKinney, D. R. Ruzicka, and R. B. Meagher (2007)
PLANT CELL 19, 3111-3126
   Abstract »    Full Text »    PDF »
The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism.
J. B. Moseley and B. L. Goode (2006)
Microbiol. Mol. Biol. Rev. 70, 605-645
   Abstract »    Full Text »    PDF »
RNase MRP Cleaves the CLB2 mRNA To Promote Cell Cycle Progression: Novel Method of mRNA Degradation.
T. Gill, T. Cai, J. Aulds, S. Wierzbicki, and M. E. Schmitt (2004)
Mol. Cell. Biol. 24, 945-953
   Abstract »    Full Text »    PDF »
The Saccharomyces cerevisiae RNase Mitochondrial RNA Processing Is Critical for Cell Cycle Progression at the End of Mitosis.
T. Cai, J. Aulds, T. Gill, M. Cerio, and M. E. Schmitt (2002)
Genetics 161, 1029-1042
   Abstract »    Full Text »    PDF »
One of the two cytoplasmic actin isoforms in Drosophila is essential.
C. R. Wagner, A. P. Mahowald, and K. G. Miller (2002)
PNAS 99, 8037-8042
   Abstract »    Full Text »    PDF »
Identification of histone H4-like TAF in Schizosaccharomyces pombe as a protein that interacts with WD repeat-containing TAF.
H. Mitsuzawa and A. Ishihama (2002)
Nucleic Acids Res. 30, 1952-1958
   Abstract »    Full Text »    PDF »
Transient Inhibition of Translation Initiation by Osmotic Stress.
Y. Uesono and A. Toh-e (2002)
J. Biol. Chem. 277, 13848-13855
   Abstract »    Full Text »    PDF »
A Targeted Gene Knockout in Drosophila.
Y. S. Rong and K. G. Golic (2001)
Genetics 157, 1307-1312
   Abstract »    Full Text »
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae.
F. Paques and J. E. Haber (1999)
Microbiol. Mol. Biol. Rev. 63, 349-404
   Abstract »    Full Text »    PDF »
Cloning-Free PCR-Based Allele Replacement Methods.
N. Erdeniz, U. H. Mortensen, and R. Rothstein (1997)
Genome Res. 7, 1174-1183
   Abstract »    Full Text »    PDF »
Chlamydomonas Inner-Arm Dynein Mutant, ida5, Has a Mutation in an Actin-encoding Gene.
T. Kato-Minoura, M. Hirono, and R. Kamiya (1997)
J. Cell Biol. 137, 649-656
   Abstract »    Full Text »    PDF »
The Yeast Gene, MDM20, Is Necessary for Mitochondrial Inheritance and Organization of the Actin Cytoskeleton.
G. J. Hermann, E. J. King, and J. M. Shaw (1997)
J. Cell Biol. 137, 141-153
   Abstract »    Full Text »    PDF »
Disruption of the yeast N-myristoyl transferase gene causes recessive lethality.
R. Duronio, D. Towler, R. Heuckeroth, and J. Gordon (1989)
Science 243, 796-800
   Abstract »    PDF »
A yeast actin-binding protein is encoded by SAC6, a gene found by suppression of an actin mutation.
A. Adams, D Botstein, and D. Drubin (1989)
Science 243, 231-233
   Abstract »    PDF »
What myosin might do.
F Solomon (1987)
Science 236, 1043-1044
   PDF »
The mitochondrial genotype can influence nuclear gene expression in yeast.
V. Parikh, M. Morgan, R Scott, L. Clements, and R. Butow (1987)
Science 235, 576-580
   Abstract »    PDF »
Expression of the Escherichia coli lacZ gene on a plasmid vector in a cyanobacterium.
J. Buzby, R. Porter, and S. Stevens Jr (1985)
Science 230, 805-807
   Abstract »    PDF »
Strategies and applications of in vitro mutagenesis.
D Botstein and D Shortle (1985)
Science 229, 1193-1201
   PDF »
An Insertional Mutation in a Transgenic Mouse Line Results in Developmental Arrest at Day 5 of Gestation.
W.H. Mark, K. Signorelli, and E. Lacy (1985)
Cold Spring Harb Symp Quant Biol 50, 453-463
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)