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Science 28 May 1982:
Vol. 216. no. 4549, pp. 999 - 1001
DOI: 10.1126/science.7043735
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Articles

Science, Vol 216, Issue 4549, 999-1001
Copyright © 1982 by American Association for the Advancement of Science

articles

Ribosomal crystalline arrays of large subunits from Escherichia coli

MW Clark, K Leonard, and JA Lake

Crystalline sheets of the 50S ribosomal subunits of Escherichia coli have been formed in vitro. Electron micrographs of these arrays diffract to 35-angstrom resolution. The lattice parameters of the crystals are a = 330 +/- 20 angstroms, b = 330 +/- 30 angstroms, and alpha = 123 degrees +/- 5 degrees, and the space group is most likely p21. These arrays of ribosomal subunits are sufficiently ordered to resolve such known features of the large ribosomal subunit as the L7/L12 stalk and the central protuberance.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A tunnel in the large ribosomal subunit revealed by three-dimensional image reconstruction.
A Yonath, K. Leonard, and H. Wittmann (1987)
Science 236, 813-816
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