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Science 21 May 1982:
Vol. 216. no. 4548, pp. 894 - 897
DOI: 10.1126/science.6177040
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Articles

Science, Vol 216, Issue 4548, 894-897
Copyright © 1982 by American Association for the Advancement of Science

articles

Transformation induced by Abelson murine leukemia virus involves production of a polypeptide growth factor

DR Twardzik, GJ Todaro, H Marquardt, FH Reynolds Jr, and Stephenson JR

Rat embryo fibroblasts transformed by Abelson murine leukemia virus (MuLV) produce and release a transforming growth factor (TGF). Production of this factor is correlated with a tyrosine-specific protein kinase that is functionally active and is associated with the major Abelson MuLV gene product, P120. Transformation-defective mutants of Abelson MuLV do not transform cells, do not have their virus coded transforming gene product phosphorylated in tyrosine, and do not induce TGF production. Abelson MuLV-induced TGF morphologically transforms cells in culture, competes with 125I-labeled epidermal growth factor (EGF) for binding to cell receptors, and induces phosphorylation of tyrosine acceptor sites in the 160,000-dalton EGF membrane receptor. After purification to homogeneity, Abelson virus-induced TGF migrates as a single polypeptide with an apparent size of 7400 daltons as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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R. Heimark, D. Twardzik, and S. Schwartz (1986)
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The action of oncogenes in the cytoplasm and nucleus.
R. Weinberg (1985)
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Rat transforming growth factor type 1: structure and relation to epidermal growth factor.
H Marquardt, M. Hunkapiller, L. Hood, and G. Todaro (1984)
Science 223, 1079-1082
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