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Science 30 April 1982:
Vol. 216. no. 4545, pp. 537 - 538
DOI: 10.1126/science.7071601
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Articles

Science, Vol 216, Issue 4545, 537-538
Copyright © 1982 by American Association for the Advancement of Science

articles

Electric pulse-induced fusion of 3T3 cells in monolayer culture

J Teissie, VP Knutson, TY Tsong, and MD Lane

Swiss mouse 3T3-C2 fibroblasts, grown to confluence in monolayer culture, are shown to fuse when exposed to electric fields. Exposure to five repetitive electric pulses of about 1 kilovolt per centimeter with a duration of 50 microseconds caused approximately 20 percent of the cells to become fused (multinucleate) when 1 millimolar magnesium was present in the medium. The effects of minimum thresholds of field strength, pulse duration, and number of pulses were determined. Cell disruption was observed when the electric field exceeded 2.0 kilovolts per centimeter or the pulse was of longer duration than 120 microseconds.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)