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Science 30 April 1982:
Vol. 216. no. 4545, pp. 522 - 523
DOI: 10.1126/science.7041257
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Articles

Science, Vol 216, Issue 4545, 522-523
Copyright © 1982 by American Association for the Advancement of Science

articles

Expression of Treponema pallidum antigens in Escherichia coli

AM Walfield, PA Hanff, and MA Lovett

Treponema pallidum DNA was cloned in a bacteriophage. Clones were screened for expression of Treponema pallidum antigens by an in situ radioimmunoassay on nitrocellulose, with the use of subsequent reactions with syphilitic serum and radioiodinated Staphylococcus aureus protein A. One clone, which gave a strong signal, codes for at least seven antigens that react specifically with human antibodies to Treponema pallidum.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Genome Scale Identification of Treponema pallidum Antigens.
M. McKevitt, M. B. Brinkman, M. McLoughlin, C. Perez, J. K. Howell, G. M. Weinstock, S. J. Norris, and T. Palzkill (2005)
Infect. Immun. 73, 4445-4450
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