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Science 2 April 1982:
Vol. 216. no. 4541, pp. 70 - 73
DOI: 10.1126/science.6175023
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Articles

Science, Vol 216, Issue 4541, 70-73
Copyright © 1982 by American Association for the Advancement of Science

articles

5-Azacytidine-induced reactivation of a herpes simplex thymidine kinase gene

DW Clough, LM Kunkel, and RL Davidson

Mouse cells transformed with herpes simplex virus and containing the viral thymidine kinase (TK) gene in an inactive state were treated with 5-azacytidine. The result was the reexpression of the viral TK gene. Two days of exposure to 5-azacytidine followed by 2 days of expression time was sufficient for maximal induction of the TK+ phenotype. The induction of TK expression by 5-azacytidine was concentration-dependent, with maximal induction at 10 micromoles per liter. 5-Azacytidine also inhibited the decay of TK expression in TK+ transformants removed from selective conditions. Analysis of the methylation patterns of the viral TK gene with restriction endonucleases Hpa II and Msp I showed the active gene to be unmethylated, the inactive gene methylated, and the 5-azacytidine-induced gene unmethylated.


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